LS-C98895

antibody from LSBio

Targeting: AURKC ARK3, AurC, STK13

Immunocytochemistry

Antibody data

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Validation data

Product number: LS-C98895 - Provider product page
Provider: LSBio
Proper citation: LifeSpan Cat#LS-C98895, RRID:AB_2061790
Product name: AURKC / Aurora C Antibody (aa1-30) LS-C98895
Antibody type: Polyclonal
Description: Ammonium sulfate precipitation
Reactivity: Human
Host: Rabbit
Storage: Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

AURKC protein structure - LS-C98895 shown in red.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: The anti-Aurora C antibody is used in Western blot to detect Aurora C in lysates of 293 cells expressing Flag tag (lane 1), Flag-tagged Aurora A (lane 2), Flag-tagged Aurora B (lane 3) or Flag-tagged Aurora C (lane 4). In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C antibody as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D). Data is kindly provided by Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: The anti-Aurora C antibody is used in Western blot to detect Aurora C in lysates of 293 cells expressing Flag tag (lane 1), Flag-tagged Aurora A (lane 2), Flag-tagged Aurora B (lane 3) or Flag-tagged Aurora C (lane 4). In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C antibody as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D). Data is kindly provided by Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Aurora-C Antibody western blot of HepG2 cell line lysates (35 ug/lane). The Aurora-C antibody detected the Aurora-C protein (arrow).