11472-1-AP

antibody from Proteintech Group

Targeting: CAPN2 CANPL2, CANPml, mCANP

Provider product page for 11472-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [25]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [1]

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Product number: 11472-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#11472-1-AP, RRID:AB_10755306
Product name: Calpain 2 antibody
Antibody type: Polyclonal
Description: KD/KO validated Calpain 2 antibody (Cat. #11472-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CAPN2 protein structure - 11472-1-AP shown in red.

Submitted references Bufei Jianspi formula alleviates intestinal epithelial barrier injury by inhibition of calpain 2 in COPD rat.

Wang K, Tao L, Zhang Q, Liu L, Shan B, Zhao P, Li J
Phytomedicine : international journal of phytotherapy and phytopharmacology 2026 May;154:158006

CAPNS1/Dicer feedback loop facilitates glycolysis, proliferation, and metastasis in hepatocellular carcinoma cells by inhibiting the maturation of MicroRNA pool.

Zeng Z, Li D, Lei S, Bao X, Ruan W, Tian Q, Tang W, Tai H, Zhang X, Hu C, Zhen Y, Chen T
Cell communication and signaling : CCS 2026 Jan 2;24(1):76

ACSL5 regulated acetyl-CoA to promote bladder cancer cellular senescence via 53BP1 acetylation.

Luo Y, Zeng Y, Liu Y, Liu S, Dai Y, Pan W, Zhang L, Zhu R, Feng D, Du K, Zhang X, Jia B, Tian F, Zhou L, Gu C
Oncogene 2025 Sep;44(34):3096-3112

MSC-EVs alleviate sepsis-induced acute lung injury through inhibiting NET-triggered damage to adherens junctions of endothelial cells.

Shao Y, Wu D, Miao C, Zhang H
Molecular immunology 2025 Sep;185:6-17

PDZD8 Dysregulation Mediates RVLM Neuronal Hyperexcitation Via Activation of Ca(2+)-Calpain-2 Signaling in Stress-Induced Hypertension.

Liu T, Wang L, Tong L, Ren Z, Zhao B, Liu H, Lu W, Zhang H, Zhang S, Du D
Molecular neurobiology 2025 Oct;62(10):12539-12558

Calpain-2-Mediated Endothelial Focal Adhesion Disruption in Thoracic Aortic Dissection.

Teng X, Wang Y, Huang H, Ding Y, Wang J, Liu M, Song K, Shao L, Yu Y, Yang Z, Shen Z
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Jul;12(25):e2501112

Calcium blockers protect against sensory epithelial damage and hearing loss in Cx26-cKO mice.

Bai X, Liao B, Hu WH, Wang MQ, Chen XB, Liu YH, Sun Y, Xu K
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2025 Dec;193:118829

Calpain inhibition as a novel therapeutic strategy for aortic dissection with acute lower extremity ischemia.

Tan Q, Wang X, Xu W, Song K, Xiong Y, Jiang Z, Li J, Yu Y, Ye W, Shen Z, Teng X
Molecular medicine (Cambridge, Mass.) 2025 Apr 21;31(1):144

Tiliroside disrupted iron homeostasis and induced ferroptosis via directly targeting calpain-2 in pancreatic cancer cells.

Xu M, Zhong W, Yang C, Liu M, Yuan X, Lu T, Li D, Zhang G, Liu H, Zeng Y, Yang X, Zhou Y, Zhou L
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 May;127:155392

CAPN2 promotes apalutamide resistance in metastatic hormone-sensitive prostate cancer by activating protective autophagy.

Qi Z, Bai X, Wu L, Zhang P, Guo Z, Yu Y
Journal of translational medicine 2024 Jun 6;22(1):538

METTL16 inhibits pancreatic cancer proliferation and metastasis by promoting MROH8 RNA stability and inhibiting CAPN2 expression - experimental studies.

Yi T, Wang C, Ye X, Lin J, Lin C, Qin F, Yang W, Ye Y, Ning D, Lan J, Li H, Luo C, Ma J, Wei Z
International journal of surgery (London, England) 2024 Dec 1;110(12):7701-7719

Calpain inhibitor MDL28170 alleviates cerebral ischemia‑reperfusion injury by suppressing inflammation and autophagy in a rat model of cardiac arrest.

Wang WY, Shi JX, Chen MH, Zhuge XZ, Dai CG, Xie L
Experimental and therapeutic medicine 2023 May;25(5):196

Fam20c regulates the calpain proteolysis system through phosphorylating Calpasatatin to maintain cell homeostasis.

Liu X, Jiang L, Zhang W, Zhang J, Luan X, Zhan Y, Wang T, Da J, Liu L, Zhang S, Guo Y, Zhang K, Wang Z, Miao N, Xie X, Liu P, Li Y, Jin H, Zhang B
Journal of translational medicine 2023 Jun 27;21(1):417

Aberrant mechanical loading induces annulus fibrosus cells apoptosis in intervertebral disc degeneration via mechanosensitive ion channel Piezo1.

Liu C, Gao X, Lou J, Li H, Chen Y, Chen M, Zhang Y, Hu Z, Chang X, Luo M, Zhai Y, Li C
Arthritis research & therapy 2023 Jul 7;25(1):117

Cardiac Myocyte-Specific Overexpression of FASTKD1 Prevents Ventricular Rupture After Myocardial Infarction.

Marshall KD, Klutho PJ, Song L, Roy R, Krenz M, Baines CP
Journal of the American Heart Association 2023 Feb 21;12(4):e025867

Zinc deficiency causes oxidative stress, endoplasmic reticulum stress, apoptosis and inflammation in hepatocytes in grass carp.

Cui J, Xu T, Lv H, Guo MY
Fish & shellfish immunology 2023 Aug;139:108905

Prominent Effects of Berbamine Hydrochloride on Alzheimer's Disease Model Mice.

Chen CL, Wang HL, Qian F, Liu MH, Guo XX, Lv J, Huang JH, Zhang NZ, Xiang Z
Frontiers in pharmacology 2022;13:939039

TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy.

Tian W, Liu SY, Zhang M, Meng JR, Tang N, Feng YD, Sun Y, Gao YY, Zhou L, Cao W, Li XQ
Pharmacological research 2022 Jul;181:106262

Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.

Wang J, Gao F, Cui S, Yang S, Gao F, Wang X, Zhu G
Pharmacological research 2022 Feb;176:106079

Mechanosensitive Piezo1 channels mediate renal fibrosis.

Zhao X, Kong Y, Liang B, Xu J, Lin Y, Zhou N, Li J, Jiang B, Cheng J, Li C, Wang W
JCI insight 2022 Apr 8;7(7)

Inhibition of Shear-Induced Platelet Aggregation by Xueshuantong via Targeting Piezo1 Channel-Mediated Ca(2+) Signaling Pathway.

Liu L, Zhang Q, Xiao S, Sun Z, Ding S, Chen Y, Wang L, Yin X, Liao F, Jiang LH, Xue M, You Y
Frontiers in pharmacology 2021;12:606245

Astrocytic Kir4.1 regulates NMDAR/calpain signaling axis in lipopolysaccharide-induced depression-like behaviors in mice.

Song Z, Bian Z, Zhang Z, Wang X, Zhu A, Zhu G
Toxicology and applied pharmacology 2021 Oct 15;429:115711

The effect of repeated bouts of electrical stimulation-induced muscle contractions on proteolytic signaling in rat skeletal muscle.

Kotani T, Takegaki J, Tamura Y, Kouzaki K, Nakazato K, Ishii N
Physiological reports 2021 May;9(9):e14842

Inhibition of extracellular signal-regulated kinase/calpain-2 pathway reduces neuroinflammation and necroptosis after cerebral ischemia-reperfusion injury in a rat model of cardiac arrest.

Wang WY, Xie L, Zou XS, Li N, Yang YG, Wu ZJ, Tian XY, Zhao GY, Chen MH
International immunopharmacology 2021 Apr;93:107377

Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.

Wang S, Huang Y, Yan Y, Zhou H, Wang M, Liao L, Wang Z, Chen D, Ji D, Xia X, Liu F, Huang J, Xiong K
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 2019 Jan;221:57-67

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Y79 cells were subjected to SDS PAGE followed by western blot with 11472-1-AP(CAPN2 antibody) at dilution of 1:200
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using CAPN2 antibody 11472-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 11472-1-AP(CAPN2 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue