14545-1-AP

antibody from Proteintech Group

Targeting: CLIC1 CLCNL1, G6, NCC27, p64CLCP

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

14545-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#14545-1-AP, RRID:AB_2081809

Product name

CLIC1 antibody

Antibody type

Polyclonal

Description

CLIC1 antibody (Cat. #14545-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

CLIC1 down-regulates Nrf2/HO-1 signalling pathway promoting the apoptosis and pyroptosis in OGD/R-treated HT22 cells.

Xiong J, Li S, Chen H, Yu C, Cao Y, Qu X, Wu J, Zhang A, Sun C
PloS one 2025;20(9):e0332698

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Circular RNA APP contributes to Alzheimer's disease pathogenesis by modulating microglial polarization via miR-1906/CLIC1 axis.

Wu DP, Wei YS, Hou LX, Du YX, Yan QQ, Liu LL, Zhao YD, Yan RY, Yu C, Zhong ZG, Huang JL
Alzheimer's research & therapy 2025 Feb 14;17(1):44

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Benzoylmesaconine mitigates NLRP3 inflammasome-related diseases by reducing intracellular K(+) efflux and disrupting NLRP3 inflammasome assembly.

Zhang Z, Wu C, Bao Z, Ren Z, Zou M, Lei S, Liu K, Deng X, Yin S, Shi Z, Zhang L, Lan Z, Chen L
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Dec;135:156154

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Extracellular Acidification Augments NLRP3-Mediated Inflammasome Signaling in Macrophages.

Chae BJ, Lee KS, Hwang I, Yu JW
Immune network 2023 Jun;23(3):e23

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Chloride intracellular channel 1 promotes esophageal squamous cell carcinoma proliferation via mTOR signalling.

Geng H, Feng C, Sun Z, Fan X, Xie Y, Gu J, Fan L, Liu G, Li C, Thorne RF, Zhang XD, Li X, Liu X
Translational oncology 2023 Jan;27:101560

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Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy.

Li S, Dong S, Shi B, Xu Q, Li L, Wang S, Zhang W, Zhong M, Zhu J, Cheng Y, Zhang G, Hu S
Oxidative medicine and cellular longevity 2022;2022:4608914

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Chloride Intracellular Channel 1 is a Potential Biomarker for Breast Cancer.

Xia J, Wang Q, Ju F, Luo X, Wang F, Zhou Y, Huang H, Wang H, Bao X
Breast cancer (Dove Medical Press) 2022;14:247-258

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Label-free LC-MS/MS proteomics analyses reveal CLIC1 as a predictive biomarker for bladder cancer staging and prognosis.

Wang W, Huang G, Lin H, Ren L, Fu L, Mao X
Frontiers in oncology 2022;12:1102392

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Chloride Intracellular Channel 1 Expression Is Associated With Poor Prognosis of Lung Adenocarcinoma.

Yasuda Y, Nagano T, Jimbo N, Kiriu T, Suraya R, Hazama D, Yamamoto M, Maniwa Y, Nishimura Y, Kobayashi K
Anticancer research 2022 Jan;42(1):271-277

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CLIC1 Drives Angiogenesis in Hepatocellular Carcinoma by Modulating VEGFA.

Wei X, Pan B, Yang M, Shu W, Khan AR, Su R, Lin H, Xu X
Technology in cancer research & treatment 2022 Jan-Dec;21:15330338221106820

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CLIC1 Inhibition Protects Against Cellular Senescence and Endothelial Dysfunction Via the Nrf2/HO-1 Pathway.

Lu D, Le Y, Ding J, Dou X, Mao W, Zhu J
Cell biochemistry and biophysics 2021 Jun;79(2):239-252

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CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation.

Tang T, Lang X, Xu C, Wang X, Gong T, Yang Y, Cui J, Bai L, Wang J, Jiang W, Zhou R
Nature communications 2017 Aug 4;8(1):202

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14545-1-AP( CLIC1 Antibody) at dilution of 1:200

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using CLIC1 antibody 14545-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 14545-1-AP(CLIC1 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 14545-1-AP(CLIC1 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue