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- References [19]
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- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 15097-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15097-1-AP, RRID:AB_2243297
- Product name
- ATP6V1B2 antibody
- Antibody type
- Polyclonal
- Description
- ATP6V1B2 antibody (Cat. #15097-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Oral Exposure to Food-Grade Nanoparticles Poses a Risk of Alzheimer's Disease-Like Symptoms by Triggering Autophagy Defects in Neurons.
E3 ligase AREL1 controls perinuclear localization of lysosomes and supports Purkinje cell survival.
FAT-5/SCD5-mediated lipid localization in lysosomes alleviates gamma radiation injury in Caenorhabditis elegans.
ATP6AP1 promotes cell proliferation and tamoxifen resistance in luminal breast cancer by inducing autophagy.
Oxr1 and Ncoa7 regulate V-ATPase to achieve optimal pH for glycosylation within the Golgi apparatus and trans-Golgi network.
TBC1D24 interacts with the v-ATPase and regulates intraorganellar pH in neurons.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder.
Acidic Nanoparticles Restore Lysosomal Acidification and Rescue Metabolic Dysfunction in Pancreatic β-Cells under Lipotoxic Conditions.
Diphyllin elicits a doubled-pronged attack on the entry of SARS-CoV-2 by inhibiting cathepsin L and furin.
Prosapogenin A induces GSDME-dependent pyroptosis of anaplastic thyroid cancer through vacuolar ATPase activation-mediated lysosomal over-acidification.
Genome-wide CRISPR screens identify ILF3 as a mediator of mTORC1-dependent amino acid sensing.
KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.
Upregulation of V-ATPase by STAT3 Activation Promotes Anoikis Resistance and Tumor Metastasis.
Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy.
The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.
Butyrate-mediated autophagy inhibition limits cytosolic Salmonella Infantis replication in the colon of pigs treated with a mixture of Lactobacillus and Bacillus.
Differential regulation of vacuolar H(+) -ATPase subunits by transforming growth factor-β1 in salivary ducts.
Proteomic identification of synaptic caspase substrates.
The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.
Shang J, Yan J, Lou H, Jiang X, Wang Z, Gao Y, Fan X, Lu X
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Jan;13(3):e08096
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Jan;13(3):e08096
E3 ligase AREL1 controls perinuclear localization of lysosomes and supports Purkinje cell survival.
Jiang L, Tang J, Zhang YF, Zou WX, Deng G, Tian N, Zhao X, Han L, Liu K, Song BL, Luo J
The EMBO journal 2026 Feb;45(3):655-691
The EMBO journal 2026 Feb;45(3):655-691
FAT-5/SCD5-mediated lipid localization in lysosomes alleviates gamma radiation injury in Caenorhabditis elegans.
Zhu T, Wang Z, Li J, Zhao Y, Fan S
The Journal of biological chemistry 2025 Oct;301(10):110674
The Journal of biological chemistry 2025 Oct;301(10):110674
ATP6AP1 promotes cell proliferation and tamoxifen resistance in luminal breast cancer by inducing autophagy.
Yan Z, Huang A, Ma D, Hong C, Zhang S, He L, Rao H, Luo S
Cell death & disease 2025 Mar 25;16(1):201
Cell death & disease 2025 Mar 25;16(1):201
Oxr1 and Ncoa7 regulate V-ATPase to achieve optimal pH for glycosylation within the Golgi apparatus and trans-Golgi network.
Yoshimura SI, Sobajima T, Kunii M, Harada A
Proceedings of the National Academy of Sciences of the United States of America 2025 Jun 3;122(22):e2505975122
Proceedings of the National Academy of Sciences of the United States of America 2025 Jun 3;122(22):e2505975122
TBC1D24 interacts with the v-ATPase and regulates intraorganellar pH in neurons.
Pepe S, Aprile D, Castroflorio E, Marte A, Giubbolini S, Hopestone S, Parsons A, Soares T, Benfenati F, Oliver PL, Fassio A
iScience 2025 Jan 17;28(1):111515
iScience 2025 Jan 17;28(1):111515
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder.
Ghoochani A, Heiby JC, Rawat ES, Medoh UN, Di Fraia D, Dong W, Gastou M, Nyame K, Laqtom NN, Gomez-Ospina N, Ori A, Abu-Remaileh M
bioRxiv : the preprint server for biology 2024 Oct 14;
bioRxiv : the preprint server for biology 2024 Oct 14;
Acidic Nanoparticles Restore Lysosomal Acidification and Rescue Metabolic Dysfunction in Pancreatic β-Cells under Lipotoxic Conditions.
Lo CH, O'Connor LM, Loi GWZ, Saipuljumri EN, Indajang J, Lopes KM, Shirihai OS, Grinstaff MW, Zeng J
ACS nano 2024 Jun 18;18(24):15452-15467
ACS nano 2024 Jun 18;18(24):15452-15467
Diphyllin elicits a doubled-pronged attack on the entry of SARS-CoV-2 by inhibiting cathepsin L and furin.
Mao B, Le-Trilling VTK, Tang H, Hu J, Schmitz MS, Barbet K, Xu D, Wei Z, Guo B, Mennerich D, Yao C, Liu J, Li Z, Wan Y, Zhang X, Wang K, Tang N, Yu Z, Trilling M, Lin Y
Virus research 2024 Dec;350:199485
Virus research 2024 Dec;350:199485
Prosapogenin A induces GSDME-dependent pyroptosis of anaplastic thyroid cancer through vacuolar ATPase activation-mediated lysosomal over-acidification.
Liu Y, Guo Y, Zeng Q, Hu Y, He R, Ma W, Qian C, Hua T, Song F, Cai Y, Zhu L, Ren X, Xu J, Zheng C, Ding L, Ge J, Wang W, Xu H, Ge M, Zheng G
Cell death & disease 2024 Aug 13;15(8):586
Cell death & disease 2024 Aug 13;15(8):586
Genome-wide CRISPR screens identify ILF3 as a mediator of mTORC1-dependent amino acid sensing.
Yan G, Yang J, Li W, Guo A, Guan J, Liu Y
Nature cell biology 2023 May;25(5):754-764
Nature cell biology 2023 May;25(5):754-764
KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.
Yan G, Li X, Zheng Z, Gao W, Chen C, Wang X, Cheng Z, Yu J, Zou G, Farooq MZ, Zhu X, Zhu W, Zhong Q, Yan X
Autophagy 2022 Dec;18(12):2799-2816
Autophagy 2022 Dec;18(12):2799-2816
Upregulation of V-ATPase by STAT3 Activation Promotes Anoikis Resistance and Tumor Metastasis.
Adeshakin FO, Adeshakin AO, Liu Z, Lu X, Cheng J, Zhang P, Yan D, Zhang G, Wan X
Journal of Cancer 2021;12(16):4819-4829
Journal of Cancer 2021;12(16):4819-4829
Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy.
Yuan L, Chu B, Chen S, Li Y, Liu N, Zhu Y, Zhou D
Microorganisms 2021 Nov 16;9(11)
Microorganisms 2021 Nov 16;9(11)
The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.
Castroflorio E, den Hoed J, Svistunova D, Finelli MJ, Cebrian-Serrano A, Corrochano S, Bassett AR, Davies B, Oliver PL
Cellular and molecular life sciences : CMLS 2021 Apr;78(7):3503-3524
Cellular and molecular life sciences : CMLS 2021 Apr;78(7):3503-3524
Butyrate-mediated autophagy inhibition limits cytosolic Salmonella Infantis replication in the colon of pigs treated with a mixture of Lactobacillus and Bacillus.
Chu B, Zhu Y, Su J, Xia B, Zou Y, Nie J, Zhang W, Wang J
Veterinary research 2020 Aug 5;51(1):99
Veterinary research 2020 Aug 5;51(1):99
Differential regulation of vacuolar H(+) -ATPase subunits by transforming growth factor-β1 in salivary ducts.
Kluge M, Namkoong E, Khakipoor S, Park K, Roussa E
Journal of cellular physiology 2019 Sep;234(9):15061-15079
Journal of cellular physiology 2019 Sep;234(9):15061-15079
Proteomic identification of synaptic caspase substrates.
Victor KG, Heffron DS, Sokolowski JD, Majumder U, Leblanc A, Mandell JW
Synapse (New York, N.Y.) 2018 Jan;72(1)
Synapse (New York, N.Y.) 2018 Jan;72(1)
The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.
Doyle T, Moncorgé O, Bonaventure B, Pollpeter D, Lussignol M, Tauziet M, Apolonia L, Catanese MT, Goujon C, Malim MH
Nature microbiology 2018 Dec;3(12):1369-1376
Nature microbiology 2018 Dec;3(12):1369-1376
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15097-1-AP(ATP6V1B2 antibody) at dilution of 1:3000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 15097-1-AP(ATP6V1B2 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The ATP6V1B2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human ATP6V1B2. This antibody recognizes human,mouse,rat antigen. The ATP6V1B2 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
