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- Antibody Data
- Antigen structure
- References [16]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- 15280-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15280-1-AP, RRID:AB_2062545
- Product name
- ATP6V1E1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated ATP6V1E1 antibody (Cat. #15280-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Calcium dysregulation disrupts mitochondrial homeostasis by interfering AMPK/Drp1 pathway to aggravate plaque progression and instability.
A heterotrimeric protein complex assembles the metazoan V-ATPase upon dissipation of proton gradients.
ATG16L1 controls mammalian vacuolar proton ATPase.
Oxr1 and Ncoa7 regulate V-ATPase to achieve optimal pH for glycosylation within the Golgi apparatus and trans-Golgi network.
Lithocholic acid binds TULP3 to activate sirtuins and AMPK to slow down ageing.
Lysosomal NKG7 restrains mTORC1 activity to promote CD8(+) T cell durability and tumor control.
Proteomic analysis links truncated tau to lysosome motility, autophagy, and endo-lysosomal dysfunction.
The ROGDI protein mutated in Kohlschutter-Tonz syndrome is a novel subunit of the Rabconnectin-3 complex implicated in V-ATPase assembly.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder.
Exploring the mechanism and phytochemicals in Psoraleae Fructus-induced hepatotoxicity based on RNA-seq, in vitro screening and molecular docking.
Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification.
Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase assembly.
Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy.
Butyrate-mediated autophagy inhibition limits cytosolic Salmonella Infantis replication in the colon of pigs treated with a mixture of Lactobacillus and Bacillus.
iTRAQ-based quantitative proteomic analysis for identification of oligodendroglioma biomarkers related with loss of heterozygosity on chromosomal arm 1p.
Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification.
Hu P, Liu M, Wu T, Zhang Y, Liu C, Wang L, Zhang W, Que Y, You J, Yu W, Tong X
Theranostics 2025;15(15):7567-7583
Theranostics 2025;15(15):7567-7583
A heterotrimeric protein complex assembles the metazoan V-ATPase upon dissipation of proton gradients.
Nardone C, Mintseris J, He D, Rutter JC, Ebert BL, Gygi SP, Rapoport T
Nature structural & molecular biology 2025 Oct;32(10):2076-2087
Nature structural & molecular biology 2025 Oct;32(10):2076-2087
ATG16L1 controls mammalian vacuolar proton ATPase.
Duque TLA, Paddar M, Trosdal E, Javed R, Allers L, Mudd MH, Akepati P, Mishra SR, Salemi M, Phinney B, Bratton SB, Wileman T, Deretic V
The Journal of cell biology 2025 Oct 6;224(10)
The Journal of cell biology 2025 Oct 6;224(10)
Oxr1 and Ncoa7 regulate V-ATPase to achieve optimal pH for glycosylation within the Golgi apparatus and trans-Golgi network.
Yoshimura SI, Sobajima T, Kunii M, Harada A
Proceedings of the National Academy of Sciences of the United States of America 2025 Jun 3;122(22):e2505975122
Proceedings of the National Academy of Sciences of the United States of America 2025 Jun 3;122(22):e2505975122
Lithocholic acid binds TULP3 to activate sirtuins and AMPK to slow down ageing.
Qu Q, Chen Y, Wang Y, Wang W, Long S, Yang HY, Wu J, Li M, Tian X, Wei X, Liu YH, Xu S, Xiong J, Yang C, Wu Z, Huang X, Xie C, Wu Y, Xu Z, Zhang C, Zhang B, Feng JW, Chen J, Feng Y, Fang H, Lin L, Xie ZK, Sun B, Tian H, Yu Y, Piao HL, Xie XS, Deng X, Zhang CS, Lin SC
Nature 2025 Jul;643(8070):201-209
Nature 2025 Jul;643(8070):201-209
Lysosomal NKG7 restrains mTORC1 activity to promote CD8(+) T cell durability and tumor control.
Ham H, Hirdler JB, Bihnam DT, Mao Z, Gicobi JK, Macedo BG, Rodriguez-Quevedo MF, Schultz DF, Correia C, Zhong J, Martinez KE, Banuelos A, Ashton DS, Lagnado AB, Guo R, Pessoa R, Pandey A, Li H, Lucien F, Borges da Silva H, Dong H, Billadeau DD
Nature communications 2025 Feb 14;16(1):1628
Nature communications 2025 Feb 14;16(1):1628
Proteomic analysis links truncated tau to lysosome motility, autophagy, and endo-lysosomal dysfunction.
Goniotaki D, Hausherr M, Lynham S, Ale A, Chennell G, Marcotti S, Marcus K, Noble W, Hanger DP, Fraser G, Srivastava DP
Alzheimer's & dementia : the journal of the Alzheimer's Association 2025 Dec;21(12):e70977
Alzheimer's & dementia : the journal of the Alzheimer's Association 2025 Dec;21(12):e70977
The ROGDI protein mutated in Kohlschutter-Tonz syndrome is a novel subunit of the Rabconnectin-3 complex implicated in V-ATPase assembly.
Winkley SR, Kane PM
The Journal of biological chemistry 2025 Apr;301(4):108381
The Journal of biological chemistry 2025 Apr;301(4):108381
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder.
Ghoochani A, Heiby JC, Rawat ES, Medoh UN, Di Fraia D, Dong W, Gastou M, Nyame K, Laqtom NN, Gomez-Ospina N, Ori A, Abu-Remaileh M
bioRxiv : the preprint server for biology 2024 Oct 14;
bioRxiv : the preprint server for biology 2024 Oct 14;
Exploring the mechanism and phytochemicals in Psoraleae Fructus-induced hepatotoxicity based on RNA-seq, in vitro screening and molecular docking.
Shang H, Liu X, Pan J, Cheng H, Ma Z, Xiao C, Gao Y
Scientific reports 2024 Jan 19;14(1):1696
Scientific reports 2024 Jan 19;14(1):1696
Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification.
Choezom D, Gross JC
Journal of cell science 2022 Mar 1;135(5)
Journal of cell science 2022 Mar 1;135(5)
Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase assembly.
Ratto E, Chowdhury SR, Siefert NS, Schneider M, Wittmann M, Helm D, Palm W
Nature communications 2022 Aug 17;13(1):4848
Nature communications 2022 Aug 17;13(1):4848
Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy.
Yuan L, Chu B, Chen S, Li Y, Liu N, Zhu Y, Zhou D
Microorganisms 2021 Nov 16;9(11)
Microorganisms 2021 Nov 16;9(11)
Butyrate-mediated autophagy inhibition limits cytosolic Salmonella Infantis replication in the colon of pigs treated with a mixture of Lactobacillus and Bacillus.
Chu B, Zhu Y, Su J, Xia B, Zou Y, Nie J, Zhang W, Wang J
Veterinary research 2020 Aug 5;51(1):99
Veterinary research 2020 Aug 5;51(1):99
iTRAQ-based quantitative proteomic analysis for identification of oligodendroglioma biomarkers related with loss of heterozygosity on chromosomal arm 1p.
Yang LS, Xu XE, Liu XP, Jin H, Chen ZQ, Liu XH, Wang Y, Huang FP, Shi Q
Journal of proteomics 2012 Dec 21;77:480-91
Journal of proteomics 2012 Dec 21;77:480-91
Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification.
Wong D, Bach H, Sun J, Hmama Z, Av-Gay Y
Proceedings of the National Academy of Sciences of the United States of America 2011 Nov 29;108(48):19371-6
Proceedings of the National Academy of Sciences of the United States of America 2011 Nov 29;108(48):19371-6
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human brain tissue were subjected to SDS PAGE followed by western blot with 15280-1-AP(ATP6V1E1 antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-ATP6V1E1 (IP:15280-1-AP, 3ug; Detection:15280-1-AP 1:500) with mouse brain tissue lysate 6000ug.
- Sample type
- tissue
Supportive validation
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The ATP6V1E1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human ATP6V1E1. This antibody recognizes human,mouse,rat antigen. The ATP6V1E1 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human testis using 15280-1-AP(ATP6V1E1 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
