18274-1-AP

antibody from Proteintech Group

Targeting: ATP6V0D1 ATP6D, ATP6DV, P39, VATX, Vma6, VPATPD

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

18274-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#18274-1-AP, RRID:AB_2258877

Product name

ATP6V0D1 antibody

Antibody type

Polyclonal

Description

ATP6V0D1 antibody (Cat. #18274-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Mycobacterium tuberculosis modulates phosphorylation of host ATP6V1E1 to promote intracellular survival.

Chen J, Tang F, Qin L, Fang W, Guan L, Wu X, Li H, Duan Y, Wang F, Peng C, Liu Z, Wang J, Huang X, Wang L, Yang H, Wang L, Sha W, Cai X, Lyu LD, Liu H, Liu F, Ge B, Zheng R
Nature communications 2026 Feb 6;17(1)

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Mitochondrial protein import stress causes lysosomal damage and progressive tissue atrophy.

Brennan NA, Wang X, Rana A, Bhagwagar SZ, Horton J, Kane PM, Chen XJ
EMBO reports 2026 Apr 27;

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Cryptotanshinone differentially induces cell death in ATP6V0D1-deficient pancreatic cancer cells.

Chen F, Lin J, Cai X, Tang H, Li S, Liang R, Kang R, Zhang Z, Tang D, Liu J
Cancer drug resistance (Alhambra, Calif.) 2025;8:45

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LAMP2-FLOT2 interaction enhances autophagosome-lysosome fusion to protect the septic heart in response to ILC2.

Shao R, Liu W, Feng Y, Guo X, Ren Z, Hou X, He B
Autophagy 2025 Sep;21(9):1888-1910

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Membrane receptors cluster phosphatidylserine to activate LC3-associated phagocytosis.

Boada-Romero E, Guy CS, Palacios G, Mari L, Poudel S, Li Z, Sharma P, Green DR
Nature cell biology 2025 Oct;27(10):1676-1687

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The deubiquitinase USP45 inhibits autophagy through actin regulation by Coronin 1B.

Lin YJ, Huang LT, Ke PY, Chen GC
The Journal of cell biology 2025 May 5;224(5)

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ATP6AP1 promotes cell proliferation and tamoxifen resistance in luminal breast cancer by inducing autophagy.

Yan Z, Huang A, Ma D, Hong C, Zhang S, He L, Rao H, Luo S
Cell death & disease 2025 Mar 25;16(1):201

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Identifying diagnostic biomarkers for glaucoma based on transcriptome combined with Mendelian randomization.

Lin X, Ma C, Zhang X, Qiu Y, Cui Y, Xu N
Scientific reports 2025 Jul 1;15(1):20695

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Control of Golgi- V-ATPase through Sac1-dependent co-regulation of PI(4)P and cholesterol.

Zhou X, van der Stoel MM, Kaptan S, Li H, Li S, Hölttä M, Vihinen H, Jokitalo E, Thiele C, Pietiläinen O, Morioka S, Sasaki J, Sasaki T, Vattulainen I, Ikonen E
Nature communications 2025 Aug 21;16(1):7808

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TMEM184B modulates endolysosomal acidification via the vesicular proton pump.

Wright EB, Larsen EG, Padilla-Rodriguez M, Langlais PR, Bhattacharya MRC
Journal of cell science 2025 Aug 1;138(15)

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The ROGDI protein mutated in Kohlschutter-Tonz syndrome is a novel subunit of the Rabconnectin-3 complex implicated in V-ATPase assembly.

Winkley SR, Kane PM
The Journal of biological chemistry 2025 Apr;301(4):108381

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Substrate-Mediated Regulation of Src Expression Drives Osteoclastogenesis Divergence.

Hu B, Chen Y, Li Y, Deng C, Niu Y, Hu Z, Li Y, Sun S, Huang Y, Deng X, Wei Y
Genes 2024 Sep 18;15(9)

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Time of exercise differentially impacts bone growth in mice.

Yu S, Tang Q, Lu X, Chen G, Xie M, Yang J, Yin Y, Zheng W, Wang J, Han Y, Zhang L, Chen L
Nature metabolism 2024 Jun;6(6):1036-1052

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Ciprofloxacin is a novel anti-ferroptotic antibiotic.

Chen F, Tang H, Lin J, Kang R, Tang D, Liu J
Heliyon 2024 Jun 15;10(11):e32571

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Alkaliptosis induction counteracts paclitaxel-resistant ovarian cancer cells via ATP6V0D1-mediated ABCB1 inhibition.

Chen F, Lin J, Kang R, Tang D, Liu J
Molecular carcinogenesis 2024 Aug;63(8):1515-1527

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Spatiotemporal roles of AMPK in PARP-1- and autophagy-dependent retinal pigment epithelial cell death caused by UVA.

Wu AY, Sekar P, Huang DY, Hsu SH, Chan CM, Lin WW
Journal of biomedical science 2023 Nov 7;30(1):91

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ATP6V0D1 promotes alkaliptosis by blocking STAT3-mediated lysosomal pH homeostasis.

Chen F, Zhu S, Kang R, Tang D, Liu J
Cell reports 2023 Jan 31;42(1):111911

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Identification of Novel Senescent Markers in Small Extracellular Vesicles.

Misawa T, Hitomi K, Miyata K, Tanaka Y, Fujii R, Chiba M, Loo TM, Hanyu A, Kawasaki H, Kato H, Maezawa Y, Yokote K, Nakamura AJ, Ueda K, Yaegashi N, Takahashi A
International journal of molecular sciences 2023 Jan 26;24(3)

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A Role for the V0 Sector of the V-ATPase in Neuroexocytosis: Exogenous V0d Blocks Complexin and SNARE Interactions with V0c.

Lévêque C, Maulet Y, Wang Q, Rame M, Rodriguez L, Mochida S, Sangiardi M, Youssouf F, Iborra C, Seagar M, Vitale N, El Far O
Cells 2023 Feb 26;12(5)

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Zinc enhances autophagic flux and lysosomal function through transcription factor EB activation and V-ATPase assembly.

Kim KR, Park SE, Hong JY, Koh JY, Cho DH, Hwang JJ, Kim YH
Frontiers in cellular neuroscience 2022;16:895750

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Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency.

Wang F, Yang Y, Boudagh G, Eskelinen EL, Klionsky DJ, Malek SN
Autophagy 2022 Aug;18(8):1982-2000

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Upregulation of V-ATPase by STAT3 Activation Promotes Anoikis Resistance and Tumor Metastasis.

Adeshakin FO, Adeshakin AO, Liu Z, Lu X, Cheng J, Zhang P, Yan D, Zhang G, Wan X
Journal of Cancer 2021;12(16):4819-4829

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The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.

Castroflorio E, den Hoed J, Svistunova D, Finelli MJ, Cebrian-Serrano A, Corrochano S, Bassett AR, Davies B, Oliver PL
Cellular and molecular life sciences : CMLS 2021 Apr;78(7):3503-3524

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DDRGK1, a crucial player of ufmylation system, is indispensable for autophagic degradation by regulating lysosomal function.

Cao Y, Li R, Shen M, Li C, Zou Y, Jiang Q, Liu S, Lu C, Li H, Liu H, Cai Y
Cell death & disease 2021 Apr 20;12(5):416

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Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.

Zhong B, Liu M, Bai C, Ruan Y, Wang Y, Qiu L, Hong Y, Wang X, Li L, Li B
Molecular therapy : the journal of the American Society of Gene Therapy 2020 Apr 8;28(4):1078-1091

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A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.

Rao VK, Zavala G, Deb Roy A, Mains RE, Eipper BA
Journal of cellular physiology 2019 Jun;234(6):8683-8697

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Disruption of the vacuolar-type H(+)-ATPase complex in liver causes MTORC1-independent accumulation of autophagic vacuoles and lysosomes.

Kissing S, Rudnik S, Damme M, Lüllmann-Rauch R, Ichihara A, Kornak U, Eskelinen EL, Jabs S, Heeren J, De Brabander JK, Haas A, Saftig P
Autophagy 2017 Apr 3;13(4):670-685

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Vacuolar ATPase in phagosome-lysosome fusion.

Kissing S, Hermsen C, Repnik U, Nesset CK, von Bargen K, Griffiths G, Ichihara A, Lee BS, Schwake M, De Brabander J, Haas A, Saftig P
The Journal of biological chemistry 2015 May 29;290(22):14166-80

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Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome.

Zeng HL, Rao X, Zhang LK, Zhao X, Zhang WP, Wang J, Xu F, Guo L
Journal of proteomics 2014 Sep 23;109:125-42

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Lysosomal storage causes cellular dysfunction in mucolipidosis II skin fibroblasts.

Otomo T, Higaki K, Nanba E, Ozono K, Sakai N
The Journal of biological chemistry 2011 Oct 7;286(40):35283-90

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse testis tissue were subjected to SDS PAGE followed by western blot with 18274-1-AP(ATP6V0D1 antibody) at dilution of 1:800

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 18274-1-AP(ATP6V0D1 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 18274-1-AP(ATP6V0D1 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue

Flow cytometry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The ATP6V0D1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human ATP6V0D1. This antibody recognizes human,mouse,rat antigen. The ATP6V0D1 antibody has been validated for the following applications: ELISA, WB, IHC, FC analysis.

Appendix

18274-1-AP.pdf

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