21634-1-AP

antibody from Proteintech Group

Targeting: SMARCA4 BAF190, BRG1, FLJ39786, hSNF2b, SNF2, SNF2-BETA, SNF2L4, SNF2LB, SWI2

Provider product page for 21634-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [54]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 21634-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#21634-1-AP, RRID:AB_10858784
Product name: SMARCA4/BRG1 antibody
Antibody type: Polyclonal
Description: KD/KO validated SMARCA4/BRG1 antibody (Cat. #21634-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

SMARCA4 protein structure - 21634-1-AP shown in red.

Submitted references SMARCA4 promotes lineage plasticity and enzalutamide resistance in prostate cancer by regulating PROX1 via H3K27 acetylation.

Wu C, Luo M, Wu C, Yuan Y, Li Y, Liao Y, Zhang Y, Huang X, Wang M, Lv S, Wei Q
Cell death discovery 2026 Mar 25;12(1)

Regulatory role of MRTF-A in macrophage polarization and the therapeutic mechanism of Bushen Wenyang Huayu decoction in endometriosis.

Meng X, Qi J, Yang X, Zhang L, Fu X, Li J, Chen J
Journal of ethnopharmacology 2026 Jul 15;366:121602

Time-resolved multiomics profiling reveals chromatin O-GlcNAc modification promotes senescence-associated transcriptional program.

Zhang N, Zhao R, Zhong X, Dong Q, Liu Y, Yu K, Han L, Meng F, Wu J, Chen Q, Li X, Chen Q, Zhang K, Huang H, Zhang J, Wu S, Ren Y, Wang W, Liu Y
Nature communications 2026 Jan 8;17(1):1406

TGFβ-activated PDHB promotes mitochondrial pyruvate metabolism and contributes to human endoderm differentiation via ATP-dependent BRG1.

Meng L, Lv J, Yi Y, Lan X, Yan C, Zhu L, Yang J, Jiang W
Nature communications 2026 Feb 17;17(1)

ZDHHC12 Palmitoylates HDAC8 to Promote the Progression of Hepatocellular Carcinoma Associated with a Diet High in Saturated Fatty Acids.

Jin X, Hong Y, Zhao Y, Shi W, Liu R, You X, Yang C, Zhang Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Oct;12(40):e05702

Lactoferrin and Osteopontin Cooperatively Promote Intestinal Epithelial Maturation in Neonatal Mice by Activating the Brg1/Notch1/Hes1 Pathway.

Zhang W, Li C, Bi R, Lu Y, Zhang Y, Shi C, Qiao Z, Sun Y, Chen J, Wang P, Wang R, Ren F, Li Y
Nutrients 2025 Oct 8;17(19)

CircNCX1 contributes to mitochondrial fusion in cardiomyocyte by mediating the expression of mitofusin 2.

Zhang H, Li J, Wang Y, Li Q, Song L, Li M
International journal of biological macromolecules 2025 Nov;329(Pt 2):147738

RECODE: a programmable guide-free C-to-U RNA editing tool.

Ichinose M, Ohta M, Shimajiri Y, Akaiwa Y, Nakamura I, Shimamoto M, Makinoda R, Ozaki S, Tamai T, Maekawa N, Tonomoto M, Nakamura T, Yagi Y, Gutmann B
Nucleic acids research 2025 Nov 26;53(22)

TGF-β2 increases eHSP90α secretion via upregulating secretory autophagy pathway.

Li J, Li J, Xie T, Huang S, Tian H, Liu M, Kong X, Zhang D, Nawaz KI, Wang M, Li H, Zhang J, Yuan F, Guo W, Cui X, Mu H, Hu Y
Cell communication and signaling : CCS 2025 Nov 12;23(1):492

Organoid drug profiling identifies methotrexate as a therapy for SCCOHT, a rare pediatric cancer.

Kim SY, de Weert TAE, Vermeulen M, Ringnalda F, Kester L, Zsiros J, Eising S, Molenaar JJ, Sanders K, van de Wetering M, Clevers H
Science advances 2025 Feb 28;11(9):eadq1724

The SWI/SNF complex mediated chromatin remodeling promotes hepatitis B virus cccDNA transcription.

Yuan X, Geng W, Wang J, Xiong C, Wu Y, Wang Y, Jin R, Wang X
Science China. Life sciences 2025 Dec;68(12):3486-3494

Construction and verification of a prognostic model for prostate cancer based on ribosome biogenesis-related genes.

Xie J, Tang Y, Wang Y, Gao X, Fu F, Liu Y, Yi C, Zhou G, Wang Z, Chen C, Jin P, Cai Y, Zhou J
BMC medical genomics 2025 Dec 19;18(1):196

Co-condensation between transcription factor and cBAF selectively modulates chromatin remodeling and gene expression.

Ye X, Xu L, Sun X, Gai Y, Yang H, Sun Q, Song J, Huang P, Zhou Q, Li Y, Li N, Jin J, Li Y, Rao W, Lu H, Wang P, Chang C, Lu Y
Nature communications 2025 Dec 11;17(1):673

SWI/SNF complex-mediated ZNF410 cooperative binding maintains chromatin accessibility and enhancer activity.

Xu S, Peng C, Ren R, Lu H, Zhao H, Xia S, Shen Y, Xu B, Zhang H, Cheng X, Blobel GA, Lan X
Cell reports 2025 Apr 22;44(4):115476

USP28 promotes PARP inhibitor resistance by enhancing SOX9-mediated DNA damage repair in ovarian cancer.

Han F, Qi G, Li R, Peng J, Yan S, Yuan C, Kong B, Ma H
Cell death & disease 2025 Apr 16;16(1):305

Impact of histone deacetylase inhibition and arimoclomol on heat shock protein expression and disease biomarkers in primary culture models of familial ALS.

Fernández Comaduran M, Minotti S, Jacob-Tomas S, Rizwan J, Larochelle N, Robitaille R, Sephton CF, Vera M, Nalbantoglu JN, Durham HD
Cell stress & chaperones 2024 Jun;29(3):359-380

SMARCA4 (BRG1) activates ABCC3 transcription to promote hepatocellular carcinogenesis.

Liu H, Yue L, Hong W, Zhou J
Life sciences 2024 Jun 15;347:122605

Methionine Promotes Milk Synthesis through the BRCC36-BRG1-mTOR Signaling Axis in Mammary Epithelial Cells.

Xie L, Li R, Zhang J, Li H, Gao X, Zhang M
Journal of agricultural and food chemistry 2024 Jan 31;72(4):2135-2144

Reactivation of the G1 enhancer landscape underlies core circuitry addiction to SWI/SNF.

Cermakova K, Tao L, Dejmek M, Sala M, Montierth MD, Chan YS, Patel I, Chambers C, Loeza Cabrera M, Hoffman D, Parchem RJ, Wang W, Nencka R, Barbieri E, Hodges HC
Nucleic acids research 2024 Jan 11;52(1):4-21

BRG1 promotes liver cancer cell proliferation and metastasis by enhancing mitochondrial function and ATP5A1 synthesis through TOMM40.

Hui Y, Leng J, Jin D, Wang G, Liu K, Bu Y, Wang Q
Cancer biology & therapy 2024 Dec 31;25(1):2375440

CircNCX1 modulates cardiomyocyte proliferation through promoting ubiquitination of BRG1.

Yijian L, Weihan S, Lin Y, Heng Z, Yu W, Lin S, Shuo M, Mengyang L, Jianxun W
Cellular signalling 2024 Aug;120:111193

ACTL6A protects gastric cancer cells against ferroptosis through induction of glutathione synthesis.

Yang Z, Zou S, Zhang Y, Zhang J, Zhang P, Xiao L, Xie Y, Meng M, Feng J, Kang L, Lee MH, Fang L
Nature communications 2023 Jul 13;14(1):4193

A program of successive gene expression in mouse one-cell embryos.

Asami M, Lam BYH, Hoffmann M, Suzuki T, Lu X, Yoshida N, Ma MK, Rainbow K, Gužvić M, VerMilyea MD, Yeo GSH, Klein CA, Perry ACF
Cell reports 2023 Feb 28;42(2):112023

Effect of mechanical unloading on genome-wide DNA methylation profile of the failing human heart.

Liao X, Kennel PJ, Liu B, Nash TR, Zhuang RZ, Godier-Furnemont AF, Xue C, Lu R, Colombo PC, Uriel N, Reilly MP, Marx SO, Vunjak-Novakovic G, Topkara VK
JCI insight 2023 Feb 22;8(4)

Regulation of 4-HNE via SMARCA4 Is Associated with Worse Clinical Outcomes in Hepatocellular Carcinoma.

Watabe S, Aruga Y, Kato R, Kawade G, Kubo Y, Tatsuzawa A, Onishi I, Kinowaki Y, Ishibashi S, Ikeda M, Fukawa Y, Akahoshi K, Tanabe M, Kurata M, Ohashi K, Kitagawa M, Yamamoto K
Biomedicines 2023 Aug 16;11(8)

SWI/SNF Blockade Disrupts PU.1-Directed Enhancer Programs in Normal Hematopoietic Cells and Acute Myeloid Leukemia.

Chambers C, Cermakova K, Chan YS, Kurtz K, Wohlan K, Lewis AH, Wang C, Pham A, Dejmek M, Sala M, Loeza Cabrera M, Aguilar R, Nencka R, Lacorazza HD, Rau RE, Hodges HC
Cancer research 2023 Apr 4;83(7):983-996

PBAF chromatin remodeler complexes that mediate meiotic transitions in mouse.

de Castro RO, Previato de Almeida L, Carbajal A, Gryniuk I, Pezza RJ
Development (Cambridge, England) 2022 Sep 15;149(18)

NICD3 regulates the expression of MUC5AC and MUC2 by recruiting SMARCA4 and is involved in the differentiation of mucinous colorectal adenocarcinoma.

Yan X, Cheng Y, Zhang X, Hu Y, Huang H, Ren J, Wen B, Yang Y, Xiao K, Hu W, Wang W
Molecular oncology 2022 Oct;16(19):3509-3532

PCGF1-PRC1 links chromatin repression with DNA replication during hematopoietic cell lineage commitment.

Takano J, Ito S, Dong Y, Sharif J, Nakajima-Takagi Y, Umeyama T, Han YW, Isono K, Kondo T, Iizuka Y, Miyai T, Koseki Y, Ikegaya M, Sakihara M, Nakayama M, Ohara O, Hasegawa Y, Hashimoto K, Arner E, Klose RJ, Iwama A, Koseki H, Ikawa T
Nature communications 2022 Nov 28;13(1):7159

Trans-activation of eotaxin-1 by Brg1 contributes to liver regeneration.

Fan Z, Kong M, Dong W, Dong C, Miao X, Guo Y, Liu X, Miao S, Li L, Chen T, Qu Y, Yu F, Duan Y, Lu Y, Zou X
Cell death & disease 2022 May 25;13(5):495

Isoleucine stimulates mTOR and SREBP-1c gene expression for milk synthesis in mammary epithelial cells through BRG1-mediated chromatin remodelling.

Hao Q, Wang Z, Wang L, Han M, Zhang M, Gao X
The British journal of nutrition 2022 May 20;:1-11

Autism-associated protein POGZ controls ESCs and ESC neural induction by association with esBAF.

Sun X, Cheng L, Sun Y
Molecular autism 2022 Jun 1;13(1):24

Glycyrrhizic Acid Attenuates Pulmonary Fibrosis of Silicosis by Inhibiting the Interaction between HMGB1 and BRG1 through PI3K/Akt/mTOR Pathway.

Niu Z, Lin J, Hao C, Xu X, Wang C, Dai K, Deng X, Deng M, Guo Y, Yao W
International journal of environmental research and public health 2022 Jul 18;19(14)

miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded multiplex RNAi at a single-cell level.

Wang D, Xiu J, Zhao J, Luo J
EMBO reports 2022 Apr 5;23(4):e53691

SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.

Hu S, Peng L, Xu C, Wang Z, Song A, Chen FX
Molecular cell 2021 Nov 4;81(21):4425-4439.e6

An epigenetic circuit controls neurogenic programs during neocortex development.

Wang A, Wang J, Tian K, Huo D, Ye H, Li S, Zhao C, Zhang B, Zheng Y, Xu L, Hua X, Wang K, Wu QF, Wu X, Zeng T, Liu Y, Zhou Y
Development (Cambridge, England) 2021 Nov 15;148(22)

Wang Y, Yang CH, Schultz AP, Sims MM, Miller DD, Pfeffer LM
Journal of cellular and molecular medicine 2021 Mar;25(6):2956-2966

SMARCB1-deficient sinonasal carcinoma: a case report and literature review.

Yanagawa N, Suzuki M, Sugimoto R, Osakabe M, Uesugi N, Shiga K, Sugai T
Journal of surgical case reports 2021 Apr;2021(4):rjab161

ADNP Controls Gene Expression Through Local Chromatin Architecture by Association With BRG1 and CHD4.

Sun X, Yu W, Li L, Sun Y
Frontiers in cell and developmental biology 2020;8:553

RUNX2 interacts with BRG1 to target CD44 for promoting invasion and migration of colorectal cancer cells.

Yan X, Han D, Chen Z, Han C, Dong W, Han L, Zou L, Zhang J, Liu Y, Chai J
Cancer cell international 2020;20:505

The chromatin remodeler Brg1 is required for formation and maintenance of hematopoietic stem cells.

Tu J, Liu X, Jia H, Reilly J, Yu S, Cai C, Liu F, Lv Y, Huang Y, Lu Z, Han S, Jiang T, Shu X, Wu X, Tang Z, Lu Q, Liu M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Sep;34(9):11997-12008

BRM-SWI/SNF chromatin remodeling complex enables functional telomeres by promoting co-expression of TRF2 and TRF1.

Wu S, Ge Y, Li X, Yang Y, Zhou H, Lin K, Zhang Z, Zhao Y
PLoS genetics 2020 Jun;16(6):e1008799

HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.

Zhu X, Lan B, Yi X, He C, Dang L, Zhou X, Lu Y, Sun Y, Liu Z, Bai X, Zhang K, Li B, Li MJ, Chen Y, Zhang L
Nucleic acids research 2020 Jul 9;48(12):6563-6582

miR-296-5p suppresses stem cell potency of hepatocellular carcinoma cells via regulating Brg1/Sall4 axis.

Shi DM, Shi XL, Xing KL, Zhou HX, Lu LL, Wu WZ
Cellular signalling 2020 Aug;72:109650

lncRNA MALAT1 binds chromatin remodeling subunit BRG1 to epigenetically promote inflammation-related hepatocellular carcinoma progression.

Huang M, Wang H, Hu X, Cao X
Oncoimmunology 2019;8(1):e1518628

Chromatin Remodeling Factor BRG1 Regulates Stemness and Chemosensitivity of Glioma Initiating Cells.

Ganguly D, Sims M, Cai C, Fan M, Pfeffer LM
Stem cells (Dayton, Ohio) 2018 Dec;36(12):1804-1815

Dysregulation of chromatin remodelling complexes in amyotrophic lateral sclerosis.

Tibshirani M, Zhao B, Gentil BJ, Minotti S, Marques C, Keith J, Rogaeva E, Zinman L, Rouaux C, Robertson J, Durham HD
Human molecular genetics 2017 Nov 1;26(21):4142-4152

The chromatin-remodeling subunit Baf200 promotes homology-directed DNA repair and regulates distinct chromatin-remodeling complexes.

de Castro RO, Previato L, Goitea V, Felberg A, Guiraldelli MF, Filiberti A, Pezza RJ
The Journal of biological chemistry 2017 May 19;292(20):8459-8471

BRG1 and BRM loss selectively impacts RB and P53, respectively: BRG1 and BRM have differential functions in vivo.

Marquez-Vilendrer SB, Rai SK, Gramling SJ, Lu L, Reisman DN
Oncoscience 2016;3(11-12):337-350

Loss of the SWI/SNF ATPase subunits BRM and BRG1 drives lung cancer development.

Marquez-Vilendrer SB, Rai SK, Gramling SJ, Lu L, Reisman DN
Oncoscience 2016;3(11-12):322-336

LncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells.

Zhu P, Wang Y, Wu J, Huang G, Liu B, Ye B, Du Y, Gao G, Tian Y, He L, Fan Z
Nature communications 2016 Dec 1;7:13608

Mechanism of BRG1 silencing in primary cancers.

Marquez-Vilendrer SB, Thompson K, Lu L, Reisman D
Oncotarget 2016 Aug 30;7(35):56153-56169

Genomic Characterization of Non-Small-Cell Lung Cancer in African Americans by Targeted Massively Parallel Sequencing.

Araujo LH, Timmers C, Bell EH, Shilo K, Lammers PE, Zhao W, Natarajan TG, Miller CJ, Zhang J, Yilmaz AS, Liu T, Coombes K, Amann J, Carbone DP
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2015 Jun 10;33(17):1966-73

The long noncoding RNA lncTCF7 promotes self-renewal of human liver cancer stem cells through activation of Wnt signaling.

Wang Y, He L, Du Y, Zhu P, Huang G, Luo J, Yan X, Ye B, Li C, Xia P, Zhang G, Tian Y, Chen R, Fan Z
Cell stem cell 2015 Apr 2;16(4):413-25

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 21634-1-AP(SMARCA4 antibody) at dilution of 1:800
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using SMARCA4 antibody 21634-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human lung cancer using 21634-1-AP(SMARCA4 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human lung cancer using 21634-1-AP(SMARCA4 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue