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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
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Validation data
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- Product number
- AP05058PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP05058PU-N, RRID:AB_1618066
- Product name
- anti ACP1 / LMW-PTPase
- Antibody type
- Polyclonal
- Antigen
- The Human protein, HCPTPA was subcloned into pRSET to express a His6 N-terminal fusion protein. This protein was expressed in E. coli and purified to homogeneity on Ni affinity matrix.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- Lot specific
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- CHO cells were transfected with either Vector (pSRa) or expression vector driving expression of HCPTPA (pSRa-LMWPTP), and harvested in Triton X-100 lysis buffer at 72 h after transfection. 2mg of lysate protein was incubated for 1 h with 10ml of sheep-anti-LMWPTP containing anti-serum, and immunoprecipitated proteins recovered by Protein A/G were separated on PAGE (12%), and HCPTPA detected using a mouse antisheep monoclonal antibody.
