AP13559PU-N

antibody from Acris Antibodies GmbH

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP13559PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP13559PU-N, RRID:AB_1748150

Product name

anti AKT1 / PKB (C-term)

Antibody type

Polyclonal

Antigen

This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region (between 446-475aa) of human AKT1.

Reactivity

Human

Host

Rabbit

Vial size

0.4 ml

Concentration

lot specific

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of AKT1 (arrow) in 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT1 gene (Lane 2) using AKT1 / PKB antibody Cat.-No. AP13559PU-N (C-term).

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of AKT1 (arrow) in Y79 cell line lysates (35ug/lane) using AKT1 / PKB antibody Cat.-No. AP13559PU-N (C-term).

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis in T47D cell line lysates (35ug/lane) using AKT1 / PKB antibody Cat.-No. AP13559PU-N (C-term). This demonstrates this antibody detected the AKT1 protein (arrow).

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram) using AKT1 / PKB antibody Cat.-No. AP13559PU-N (C-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.