Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- R1467 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1467, RRID:AB_972621
- Product name
- anti AKT2 / PKB beta
- Antibody type
- Polyclonal
- Antigen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide of human AKT2 conjugated to Keyhole Limpet Hemocyanin (KLH).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Immunoblotting. Affinity Purified antibody to AKT2 was used at a 1:1,000 dilution to detect AKT2 by Western blot. A lysate from a stable HEK293 cell line expressing an inducible, myristoylated form of Akt2 (MyrAkt2-ER) was loaded for SDSPAGE, separated and then transferred to nitrocellulose. The blot was reacted with primary antibody for 1h at room temperature. In response to 4OHT (tamoxifen), the Akt2 is recruited to the plasma membrane via its myristoylation sequence and becomes phosphorylated and activated. The blot (right panel) shows 0 m, 15 m, and 30 m of 4OHT treatment. This treatment has no effect on endogenous Akt2, but causes a band shift upwards in the MyrAkt2. Endogenous Akt2 runs at about 60kDa whereas the myristoylated construct runs at around 110 kDa.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 2. Immunoblotting. Affinity Purified antibody to AKT2 was used at a 1:1,000 dilution to detect AKT2 by Western blot in lysates from mouse tissues. The antibody is shown to react with mouse Akt2 in mouse liver, skeletal muscle and fat using standard immunoblotting methods.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 3. Immunoblotting. Antibody to AKT2 detects both unphosphorylated and phosphorylated AKT2. As before approximately 20 mg/lane of crude HEK293 lysate was loaded for SDS-PAGE, separated and then transferred to nitrocellulose. The right lane contains lysate pretreated for 15 min with 25 uM LY294002 which affects the phosphorylation of endogenous Akt2 but has no effect on phosphorylation of the myristoylated construct. This antibody clearly detects both the phosphorylated (top arrow) and the non-phosphorylated (bottom arrow) forms of endogenous Akt2.
