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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- DM3523P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#DM3523P, RRID:AB_1008482
- Product name
- anti CD309 / VEGFR-2 / Flk-1
- Antibody type
- Monoclonal
- Antigen
- Recombinant Human soluble extracellular VEGFR-2 / KDR (D7) (110 kDa) protein (Cat.-No AR26018PU-N).
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4 (20I6)
- Vial size
- 0.1 mg
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of immunoprecipitated VEGFR-2/KDR from total lysate of HUVECs using anti-human VEGFR-2 Clone 4 (DM3523P)
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Up-regulation of VEGFR-2 in primary HUVECs by bFGF: Freshly isolated HUVECs (passage 1) were cultured in EBM. Subconfluent cultures were stimulated with VEGF (5 ng/ml) or bFGF (10 ng/ml) for 3 days. Total lysate was prepared and subjected to immunoprecipitation (anti-human VEGFR-2 Antibody Cat.-No DM3522P) followed by Western blotting (anti-human VEGFR-2 antibody Cat.-No DM3523P). (Bernhard Barleon et.al., unpublished data!).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Recombinant human endogenous soluble VEGFR-2/KDR (esKDR) was produced in insect cells. Western blot was performed using our monoclonal anti-VEGFR-2 #4 (Cat.-No DM3523P) recognizing the soluble as well as the transmembrane form of KDR (left panal) ad the new poyclonal antibody (Cat.-No AP26034PU-L) directed against the unique C-terminal end of the endogenous sKDR (CGRETILDHSAEAVGMP) recognizing solely the endogenous form but not sKDR (D7) and sKDR (D7)-Fc consisting of the full extraplasmatic domain. The endogenous sKDR generated by alternative splicing consists of the first 6 Ig-like loops with a unique c-terminal end.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Up-regulation of VEGFR-2 in vein ECs of an intact Human umbilical cord by bFGF: A fresh human umbilical cord was rinsed with PBS to remove residual blood cells, cut in small pieces (about 0.5 cm), incubated in EBM (1% FCS) and stimulated with or without 20 ng/ml bFGF for 24 h. Pieces were frozen in liquid nitrogen and used for immunohistochemistry using the mab anti-human VEGFR-2 antibody Cat.-No DM3523P as detection antibody. (Bernhard Barleon et al., unpublished data!)
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- FACS analysis of VEGFR-2/KDR expression in HUVE cells [5μg/ml #DM3523P; 5μg/ml PE goat anti-mouse IgG]. The experiment was performed by Trisha M. Westerhof, University of California, Irvine.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).
