H00002026-M01

antibody from Abnova Corporation

Targeting: ENO2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002026-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002026-M01, RRID:AB_489725

Product name

ENO2 monoclonal antibody (M01), clone 1A3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant ENO2.

Antigen sequence

PKRIERAVEEKACNCLLLKVNQIGSVTEAIQACKL
AQENGWGVMVSHRSGETEDTFIADLVVGLCTGQIK
TGAPCRSERLAKYNQLMRIEEELGDEARFAGHNFR
NPSVL


Isotype

IgG

Antibody clone number

1A3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Identification of alpha-enolase as an autoantigen in lung cancer: its overexpression is associated with clinical outcomes.

Chang GC, Liu KJ, Hsieh CL, Hu TS, Charoenfuprasert S, Liu HK, Luh KT, Hsu LH, Wu CW, Ting CC, Chen CY, Chen KC, Yang TY, Chou TY, Wang WH, Whang-Peng J, Shih NY
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Oct 1;12(19):5746-54

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ENO2 monoclonal antibody (M01), clone 1A3. Western Blot analysis of ENO2 expression in different cell lines.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ENO2 expression in transfected 293T cell line by ENO2 monoclonal antibody (M01), clone 1A3.Lane 1: ENO2 transfected lysate(47.3 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ENO2 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol