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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB0424 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0424, RRID:AB_982391
- Product name
- CASP9 (phospho S196) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CASP9.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references c-Abl tyrosine kinase regulates caspase-9 autocleavage in the apoptotic response to DNA damage.
Apoptosis of hematopoietic cells induced by growth factor withdrawal is associated with caspase-9 mediated cleavage of Raf-1.
Protein kinase A regulates caspase-9 activation by Apaf-1 downstream of cytochrome c.
Raina D, Pandey P, Ahmad R, Bharti A, Ren J, Kharbanda S, Weichselbaum R, Kufe D
The Journal of biological chemistry 2005 Mar 25;280(12):11147-51
The Journal of biological chemistry 2005 Mar 25;280(12):11147-51
Apoptosis of hematopoietic cells induced by growth factor withdrawal is associated with caspase-9 mediated cleavage of Raf-1.
Cornelis S, Bruynooghe Y, Van Loo G, Saelens X, Vandenabeele P, Beyaert R
Oncogene 2005 Feb 24;24(9):1552-62
Oncogene 2005 Feb 24;24(9):1552-62
Protein kinase A regulates caspase-9 activation by Apaf-1 downstream of cytochrome c.
Martin MC, Allan LA, Lickrish M, Sampson C, Morrice N, Clarke PR
The Journal of biological chemistry 2005 Apr 15;280(15):15449-55
The Journal of biological chemistry 2005 Apr 15;280(15):15449-55
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The CASP9 (phospho S196) polyclonal antibody (Cat # PAB0424) is used in Western blot to detect Phospho-CASP9-S196 in 293 (left) and Y-79 (right) cell lysates.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CASP9 (phospho S196) polyclonal antibody (Cat # PAB0424) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
