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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PAB0444 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0444, RRID:AB_982458
- Product name
- JUN (phospho S63) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of JUN.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification of endogenous glucocorticoid repressed genes differentially regulated by a glucocorticoid receptor mutant able to separate between nuclear factor-kappaB and activator protein-1 repression.
Ultraviolet irradiation induces Smad7 via induction of transcription factor AP-1 in human skin fibroblasts.
Differential regulation of c-Jun-dependent transcription by SUMO-specific proteases.
Bladh LG, Lidén J, Dahlman-Wright K, Reimers M, Nilsson S, Okret S
Molecular pharmacology 2005 Mar;67(3):815-26
Molecular pharmacology 2005 Mar;67(3):815-26
Ultraviolet irradiation induces Smad7 via induction of transcription factor AP-1 in human skin fibroblasts.
Quan T, He T, Voorhees JJ, Fisher GJ
The Journal of biological chemistry 2005 Mar 4;280(9):8079-85
The Journal of biological chemistry 2005 Mar 4;280(9):8079-85
Differential regulation of c-Jun-dependent transcription by SUMO-specific proteases.
Cheng J, Perkins ND, Yeh ET
The Journal of biological chemistry 2005 Apr 15;280(15):14492-8
The Journal of biological chemistry 2005 Apr 15;280(15):14492-8
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of JUN (phospho S63) polyclonal antibody (Cat # PAB0444) in mouse brain tissue lysate (35 ug/lane). Mouse Phospho-JUN-pS63 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with JUN (phospho S63) polyclonal antibody (Cat # PAB0444) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot blot analysis of JUN (phospho S63) polyclonal antibody (Cat # PAB0444) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)
