PAB0606

antibody from Abnova Corporation

Targeting: RPS6KB1 p70(S6K)-alpha, PS6K, S6K, S6K1, STK14A

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB0606

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB0606, RRID:AB_982783

Product name

RPS6KB1 (phospho S418) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of RPS6KB1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

ERBB2, TBX2, RPS6KB1, and MYC alterations in breast tissues of BRCA1 and BRCA2 mutation carriers.

Adem C, Soderberg CL, Hafner K, Reynolds C, Slezak JM, Sinclair CS, Sellers TA, Schaid DJ, Couch F, Hartmann LC, Jenkins RB
Genes, chromosomes & cancer 2004 Sep;41(1):1-11

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Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions.

Suzuki Y, Yamashita R, Shirota M, Sakakibara Y, Chiba J, Mizushima-Sugano J, Nakai K, Sugano S
Genome research 2004 Sep;14(9):1711-8

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Ribosomal p70S6K basal activity increases upon induction of differentiation of myelomonocytic leukemic cell lines HL60, AML14 and MPD.

Gomez-Cambronero J, Frye T, Baumann M
Leukemia research 2004 Jul;28(7):755-62

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

RPS6KB1 (phospho S418) polyclonal antibody (Cat # PAB0606). Western blot analysis of RPS6KB1 expression in MCF-7.

Other assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Dot blot analysis of RPS6KB1 (phospho S418) polyclonal antibody (Cat # PAB0606) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.

Validation comment

Dot Blot (Peptide)