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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PAB0608 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0608, RRID:AB_982537
- Product name
- ESPL1 (phospho S1126) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of ESPL1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Processing, localization, and requirement of human separase for normal anaphase progression.
Caspase proteolysis of the cohesin component RAD21 promotes apoptosis.
Regulation of human separase by securin binding and autocleavage.
Chestukhin A, Pfeffer C, Milligan S, DeCaprio JA, Pellman D
Proceedings of the National Academy of Sciences of the United States of America 2003 Apr 15;100(8):4574-9
Proceedings of the National Academy of Sciences of the United States of America 2003 Apr 15;100(8):4574-9
Caspase proteolysis of the cohesin component RAD21 promotes apoptosis.
Chen F, Kamradt M, Mulcahy M, Byun Y, Xu H, McKay MJ, Cryns VL
The Journal of biological chemistry 2002 May 10;277(19):16775-81
The Journal of biological chemistry 2002 May 10;277(19):16775-81
Regulation of human separase by securin binding and autocleavage.
Waizenegger I, Giménez-Abián JF, Wernic D, Peters JM
Current biology : CB 2002 Aug 20;12(16):1368-78
Current biology : CB 2002 Aug 20;12(16):1368-78
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with ESPL1 (phospho S1126) polyclonal antibody (Cat # PAB0608) which was peroxidase-conjugated to the secondary antibody followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot blot analysis of ESPL1 (phospho S1126) polyclonal antibody (Cat # PAB0608) on nitrocellulose membrane. 50 ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)
