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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- PAB3536 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3536, RRID:AB_1572400
- Product name
- CDC2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CDC2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Mitosis persists in the absence of Cdk1 activity when proteolysis or protein phosphatase activity is suppressed.
Differential repression of c-myc and cdc2 gene expression by ERF and PE-1/METS.
Control of mitotic exit by PP2A regulation of Cdc25C and Cdk1.
Skoufias DA, Indorato RL, Lacroix F, Panopoulos A, Margolis RL
The Journal of cell biology 2007 Nov 19;179(4):671-85
The Journal of cell biology 2007 Nov 19;179(4):671-85
Differential repression of c-myc and cdc2 gene expression by ERF and PE-1/METS.
Hester KD, Verhelle D, Escoubet-Lozach L, Luna R, Rose DW, Glass CK
Cell cycle (Georgetown, Tex.) 2007 Jul 1;6(13):1594-604
Cell cycle (Georgetown, Tex.) 2007 Jul 1;6(13):1594-604
Control of mitotic exit by PP2A regulation of Cdc25C and Cdk1.
Forester CM, Maddox J, Louis JV, Goris J, Virshup DM
Proceedings of the National Academy of Sciences of the United States of America 2007 Dec 11;104(50):19867-72
Proceedings of the National Academy of Sciences of the United States of America 2007 Dec 11;104(50):19867-72
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CDC2 (arrow) using rabbit CDC2 polyclonal antibody (Cat # PAB3536). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDC2 gene (Lane 2) (Origene Technologies).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CDC2 polyclonal antibody (Cat # PAB3536) in 293 cell line lysates (35 ug/lane). CDC2 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of 293 cells using CDC2 polyclonal antibody (Cat # PAB3536)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
