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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB6243 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6243, RRID:AB_1580128
- Product name
- RPS6KB1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of RPS6KB1.
- Antigen sequence
C-MISKRPEHLRMNL- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Cloning and expression of two human p70 S6 kinase polypeptides differing only at their amino termini.
Grove JR, Banerjee P, Balasubramanyam A, Coffer PJ, Price DJ, Avruch J, Woodgett JR
Molecular and cellular biology 1991 Nov;11(11):5541-50
Molecular and cellular biology 1991 Nov;11(11):5541-50
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- RPS6KB1 polyclonal antibody (Cat # PAB6243) staining (0.2 ug/mL) of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- RPS6KB1 polyclonal antibody (Cat # PAB6243)(2 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
