PAB8131

antibody from Abnova Corporation

Targeting: PDPK1 PDK1

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB8131

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB8131, RRID:AB_1710836

Product name

PDPK1 (phospho S396) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of PDPK1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Peroxisomal targeting as a tool for assaying potein-protein interactions in the living cell: cytokine-independent survival kinase (CISK) binds PDK-1 in vivo in a phosphorylation-dependent manner.

Nilsen T, Slagsvold T, Skjerpen CS, Brech A, Stenmark H, Olsnes S
The Journal of biological chemistry 2004 Feb 6;279(6):4794-801

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In vivo role of the PIF-binding docking site of PDK1 defined by knock-in mutation.

Collins BJ, Deak M, Arthur JS, Armit LJ, Alessi DR
The EMBO journal 2003 Aug 15;22(16):4202-11

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Membrane localization of 3-phosphoinositide-dependent protein kinase-1 stimulates activities of Akt and atypical protein kinase C but does not stimulate glucose transport and glycogen synthesis in 3T3-L1 adipocytes.

Egawa K, Maegawa H, Shi K, Nakamura T, Obata T, Yoshizaki T, Morino K, Shimizu S, Nishio Y, Suzuki E, Kashiwagi A
The Journal of biological chemistry 2002 Oct 11;277(41):38863-9

Other assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Dot blot analysis of PDPK1 (phospho S396) polyclonal antibody (Cat # PAB8131) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.

Validation comment

Dot Blot (Peptide)