H00009252-D01P

antibody from Abnova Corporation

Targeting: RPS6KA5 MSK1, RLPK

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00009252-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00009252-D01P, RRID:AB_1710155

Product name

RPS6KA5 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human RPS6KA5 protein.

Antigen sequence

MEEEGGSSGGAAGTSADGGDGGEQLLTVKHELRTA
NLTGHAEKVGIENFELLKVLGTGAYGKVFLVRKIS
GHDTGKLYAMKVLKKATIVQKAKTTEHTRTERQVL
EHIRQSPFLVTLHYAFQTETKLHLILDYINGGELF
THLSQRERFTEHEVQIYVGEIVLALEHLHKLGIIY
RDIKLENILLDSNGHVVLTDFGLSKEFVADETERA
YSFCGTIEYMAPDIVRGGDSGHDKAVDWWSLGVLM
YELLTGASPFTVDGEKNSQAEISRRILKSEPPYPQ
EMSALAKDLIQRLLMKDPKKRLGCGPRDADEIKEH
LFFQKINWDDLAAKKVPAPFKPVIRDELDVSNFAE
EFTEMDPTYSPAALPQSSEKLFQGYSFVAPSILFK
RNAAVIDPLQFHMGVERPGVTNVARSAMMKDSPFY
QHYDLDLKDKPLGEGSFSICRKCVHKKSNQAFAVK
IISKRMEANTQKEITALKLCEGHPNIVKLHEVFHD
QLHTFLVMELLNGGELFERIKKKKHFSETEASYIM
RKLVSAVSHMHDVGVVHRDLKPENLLFTDENDNLE
IKIIDFGFARLKPPDNQPLKTPCFTLHYAAPELLN
QNGYDESCDLWSLGVILYTMLSGQVPFQSHDRSLT
CTSAVEIMKKIKKGDFSFEGEAWKNVSQEAKDLIQ
GLLTVDPNKRLKMSGLRYNEWLQDGSQLSSNPLMT
PDILGSSGAAVHTCVKATFHAFNKYKREGFCLQNV
DKAPLAKRRKMKKTSTSTETRSSSSESSHSSSSHS
HGKTTPTKTLQPSNPADSNNPETLFQFSDSVA


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RPS6KA5 expression in transfected 293T cell line (H00009252-T02) by RPS6KA5 MaxPab polyclonal antibody.Lane 1: RPS6KA5 transfected lysate(89.90 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

RPS6KA5 MaxPab rabbit polyclonal antibody. Western Blot analysis of RPS6KA5 expression in rat brain.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between RPS6KA5 and EGF. HeLa cells were stained with anti-RPS6KA5 rabbit purified polyclonal 1:1200 and anti-EGF mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)