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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB9993 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9993, RRID:AB_1707318
- Product name
- CREB1 (phospho S133) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CREB1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references MSK1 is required for CREB phosphorylation in response to mitogens in mouse embryonic stem cells.
Distinct cAMP response element-binding protein (CREB) domains stimulate different steps in a concerted mechanism of transcription activation.
CREB regulates MHC class II expression in a CIITA-dependent manner.
Arthur JS, Cohen P
FEBS letters 2000 Sep 29;482(1-2):44-8
FEBS letters 2000 Sep 29;482(1-2):44-8
Distinct cAMP response element-binding protein (CREB) domains stimulate different steps in a concerted mechanism of transcription activation.
Kim J, Lu J, Quinn PG
Proceedings of the National Academy of Sciences of the United States of America 2000 Oct 10;97(21):11292-6
Proceedings of the National Academy of Sciences of the United States of America 2000 Oct 10;97(21):11292-6
CREB regulates MHC class II expression in a CIITA-dependent manner.
Moreno CS, Beresford GW, Louis-Plence P, Morris AC, Boss JM
Immunity 1999 Feb;10(2):143-51
Immunity 1999 Feb;10(2):143-51
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CREB1 (phospho S133) polyclonal antibody (Cat # PAB9993) was used to detect phosphorylated CREB1 by immunoblot.Recombinant His-tagged human CREB1 was produced in E. coli and purified by metal affinity chromatography.An aliquot of purified CREB1 was phosphorylated in-vitro using Protein Kinase A and ATP.Immunoblot of control (-) and in-vitro phosphorylated CREB1 (+) was used to show that the antibody reacts specifically with the phosphorylated form.CREB1 polyclonal antibody (Cat # PAB9994) reacts equally with both non-phosphorylated and phosphorylated CREB1 (not shown).Detection occurs using a 1 : 500 dilution of antibody followed by 1 : 5,000 dilution of HRP Goat-a-Rabbit IgG with visualization via ECL.Film exposure was approximately 1'.Personal Communication, Boss, J., Emory University School of Medicine, Atlanta, GA.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CREB1 (phospho S133) polyclonal antibody (Cat # PAB9993) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.This image shows moderate to strong nuclear staining of tonsillar lymphocytes.Tissue was formalin-fixed and paraffin embedded.The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
