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LearnPAB10278
antibody from Abnova Corporation
Targeting: MTOR
FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- PAB10278 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10278, RRID:AB_1676368
- Product name
- MTOR polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MTOR.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Stimulation of signal transducer and activator of transcription-1 (STAT1)-dependent gene transcription by lipopolysaccharide and interferon-gamma is regulated by mammalian target of rapamycin.
The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif.
Phospholipase D confers rapamycin resistance in human breast cancer cells.
Kristof AS, Marks-Konczalik J, Billings E, Moss J
The Journal of biological chemistry 2003 Sep 5;278(36):33637-44
The Journal of biological chemistry 2003 Sep 5;278(36):33637-44
The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif.
Nojima H, Tokunaga C, Eguchi S, Oshiro N, Hidayat S, Yoshino K, Hara K, Tanaka N, Avruch J, Yonezawa K
The Journal of biological chemistry 2003 May 2;278(18):15461-4
The Journal of biological chemistry 2003 May 2;278(18):15461-4
Phospholipase D confers rapamycin resistance in human breast cancer cells.
Chen Y, Zheng Y, Foster DA
Oncogene 2003 Jun 19;22(25):3937-42
Oncogene 2003 Jun 19;22(25):3937-42
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Supportive validation
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- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using MTOR polyclonal antibody (Cat # PAB10278) shows detection of a band ~ 245 KDa corresponding to human MTOR (arrowhead).Approximately 30 ug of HEK293 cell lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose.After blocking, the membrane was probed with the primary antibody diluted to 1 : 650 for 2h at RT.The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
