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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- MAB2347 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2347, RRID:AB_10631626
- Product name
- ESPL1 monoclonal antibody, clone XJ11-4D7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant ESPL1.
- Isotype
- IgG
- Antibody clone number
- XJ11-4D7
- Storage
- Store at -20°C or -80°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Functional interaction between BubR1 and securin in an anaphase-promoting complex/cyclosomeCdc20-independent manner.
Regulation of the anaphase-promoting complex-separase cascade by transforming growth factor-beta modulates mitotic progression in bone marrow stromal cells.
Discordant proliferation and differentiation in pituitary tumor-transforming gene-null bone marrow stem cells.
Processing, localization, and requirement of human separase for normal anaphase progression.
Kim HS, Jeon YK, Ha GH, Park HY, Kim YJ, Shin HJ, Lee CG, Chung DH, Lee CW
Cancer research 2009 Jan 1;69(1):27-36
Cancer research 2009 Jan 1;69(1):27-36
Regulation of the anaphase-promoting complex-separase cascade by transforming growth factor-beta modulates mitotic progression in bone marrow stromal cells.
Fujita T, Epperly MW, Zou H, Greenberger JS, Wan Y
Molecular biology of the cell 2008 Dec;19(12):5446-55
Molecular biology of the cell 2008 Dec;19(12):5446-55
Discordant proliferation and differentiation in pituitary tumor-transforming gene-null bone marrow stem cells.
Rubinek T, Chesnokova V, Wolf I, Wawrowsky K, Vlotides G, Melmed S
American journal of physiology. Cell physiology 2007 Sep;293(3):C1082-92
American journal of physiology. Cell physiology 2007 Sep;293(3):C1082-92
Processing, localization, and requirement of human separase for normal anaphase progression.
Chestukhin A, Pfeffer C, Milligan S, DeCaprio JA, Pellman D
Proceedings of the National Academy of Sciences of the United States of America 2003 Apr 15;100(8):4574-9
Proceedings of the National Academy of Sciences of the United States of America 2003 Apr 15;100(8):4574-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Overlay (blue) of centrosomal staining in HeLa cells. Centrosomal staining of ESPL1 (yellow), using ESPL1 monoclonal antibody, clone XJ11-4D7 (Cat # MAB2347), and gamma tubulin (green) in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells.
- Validation comment
- Immunofluorescence
