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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Other assay [1]
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Validation data
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- Product number
- PAB12654 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12654, RRID:AB_10550851
- Product name
- AR (phospho S210) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of AR.
- Antigen sequence
EASGA- Storage
- Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Androgen receptor gene knockout male mice exhibit impaired cardiac growth and exacerbation of angiotensin II-induced cardiac fibrosis.
Ikeda Y, Aihara K, Sato T, Akaike M, Yoshizumi M, Suzaki Y, Izawa Y, Fujimura M, Hashizume S, Kato M, Yagi S, Tamaki T, Kawano H, Matsumoto T, Azuma H, Kato S, Matsumoto T
The Journal of biological chemistry 2005 Aug 19;280(33):29661-6
The Journal of biological chemistry 2005 Aug 19;280(33):29661-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- 5 mg of the whole cell lysate derived from conditioned LNCaP were immunoprecipitated by 4 ug of AR (phospho S210) polyclonal antibody (Cat # PAB12654, lane 1) or pre adsorbed by immunization peptide (lane 2) followed by AR polyclonal antibody (Cat # PAB12655) at 1 : 500.
- Validation comment
- Immunoprecipitation
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot Blot : 1 ug peptide was blot onto NC membrane.A : AR (pS210).B : AR (Non-phospho).Followed were blotted at a 1 : 2000 dilution by AR (phospho S210) polyclonal antibody (Cat # PAB12654).
- Validation comment
- Dot Blot (Peptide)
