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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- MAB3485 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB3485, RRID:AB_10547406
- Product name
- CASP2 monoclonal antibody, clone 11B4
- Antibody type
- Monoclonal
- Description
- Rat monoclonal antibody raised against partial recombinant CASP2.
- Isotype
- IgG
- Antibody clone number
- 11B4
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection of pro-CASP2 (p51) , an intermediate cleavage product of CASP2 (p32) and activated CASP2 (p19) in FDC-P1 cells (mouse IL-3 dependent promyelocytic line) during growth factor with drawal induced apoptosis. Activation of CASP2 is inhibited in the presence of the caspase inhibitor Z-VAD-FMK.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of cell lysates from human and mouse cell lines with CASP2 monoclonal antibody, clone 11B4 (Cat # MAB3485). Clone 11B4 demonstrates its ability to recognize an approximately 51 kDa protein that corresponds to endogenous pro-CASP2. Hsp-70 was used as an equal loading control, and -/- CASP2 cells were used as a negative control.
