H00003725-B01P

antibody from Abnova Corporation

Targeting: JUN AP-1, c-Jun

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003725-B01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003725-B01P, RRID:AB_10551765

Product name

JUN purified MaxPab mouse polyclonal antibody (B01P)

Antibody type

Polyclonal

Description

Mouse polyclonal antibody raised against a full-length human JUN protein.

Antigen sequence

MTAKMETTFYDDALNASFLPSESGPYGYSNPKILK
QSMTLNLADPVGSLKPHLRAKNSDLLTSPDVGLLK
LASPELERLIIQSSNGHITTTPTPTQFLCPKNVTD
EQEGFAEGFVRALAELHSQNTLPSVTSAAQPVNGA
GMVAPAVASVAGGSGSGGFSASLHSEPPVYANLSN
FNPGALSSGGGAPSYGAAGLAFPAQPQQQQQPPHH
LPQQMPVQHPRLQALKEEPQTVPEMPGETPPLSPI
DMESQERIKAERKRMRNRIAASKCRKRKLERIARL
EEKVKTLKAQNSELASTANMLREQVAQLKQKVMNH
VNSGCQLMLTQQLQTF


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of JUN expression in transfected 293T cell line (H00003725-T01) by JUN MaxPab polyclonal antibody.Lane 1: JUN transfected lysate(36.41 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between GSK3B and JUN. HeLa cells were stained with anti-GSK3B rabbit purified polyclonal 1:1200 and anti-JUN mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)