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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB6901 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB6901, RRID:AB_10678209
- Product name
- CD19 monoclonal antibody, clone BU12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against native CD19.
- Isotype
- IgG
- Antibody clone number
- BU12
- Storage
- Store at 4°C.
Submitted references The CD19/CR2/TAPA-1 complex of B lymphocytes: linking natural to acquired immunity.
Engaging CD19 or target of an antiproliferative antibody 1 on human B lymphocytes induces binding of B cells to the interfollicular stroma of human tonsils via integrin alpha 4/beta 1 and fibronectin.
Fearon DT, Carter RH
Annual review of immunology 1995;13:127-49
Annual review of immunology 1995;13:127-49
Engaging CD19 or target of an antiproliferative antibody 1 on human B lymphocytes induces binding of B cells to the interfollicular stroma of human tonsils via integrin alpha 4/beta 1 and fibronectin.
Behr S, Schriever F
The Journal of experimental medicine 1995 Nov 1;182(5):1191-9
The Journal of experimental medicine 1995 Nov 1;182(5):1191-9
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- 5 X 105 cultured Raji cells were washed and incubated 45 minutes on ice with 80 uL of CD19 monoclonal antibody, clone BU12 (Cat # MAB6901) at 10 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACstar plus. Cells stained positive with a mean shift of 1.58 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
- Validation comment
- Flow Cytometry
