TA503250

antibody from OriGene

Targeting: ACY1

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503250

Provider

OriGene

Proper citation

OriGene Cat#TA503250, RRID:AB_11127790

Product name

ACY1 (Aminoacylase 1) mouse monoclonal antibody, clone OTI1H9 (formerly 1H9)

Antibody type

Monoclonal

Description

ACY1 (Aminoacylase 1) mouse monoclonal antibody, clone OTI1H9 (formerly 1H9)

Host

Mouse

Conjugate

Unconjugated

Epitope

ACY1

Isotype

IgG

Antibody clone number

OTI1H9

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY1 (RC201284, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 1 cell line by using anti-ACY1 monoclonal antibody.(1:200)

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC201284 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACY1 antibody(TA503250), and then analyzed by flow cytometry.

Validation comment

FC