600-401-425

antibody from Rockland Immunochemicals, Inc.

Targeting: AKT2 PKBβ

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-425

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-425, RRID:AB_2242524

Product name

Anti-AKT2 (RABBIT) Antibody - 600-401-425

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western Blot of Rabbit Anti-AKT2 antibody. Lane 1: 0 min. Lane 2: 15 min 4OHT (tamoxifen). Lane 3: 30 min 4OHT (tamoxifen). Lane 4: 0 min lysate pretreated-for-15min-with 25uM LY294002. Lane 5: 15 min 4OHT (tamoxifen) lysate pretreated-for-15min-with 25uM LY294002. Lane 6: 30 min 4OHT (tamoxifen) lysate pretreated-for-15min-with 25uM LY294002. Load: 20mg/lane crude HEK293 lysate. Primary antibody: Anti-AKT2 Antibody at 1:1000 for 1h at room temperature. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Observed size: Endogenous Akt2 ~60kDa, myristoylated construct ~110kDa. Other band(s): This treatment has no effect on endogenous Akt2, but causes a band shift upwards in the MyrAkt2.

Validation comment

Western Blot