321608

antibody from BioLegend

Targeting: CCR5 CC-CKR-5, CD195, CKR-5, CKR5, CMKBR5, IDDM22

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

321608

Provider

BioLegend

Proper citation

BioLegend Cat#321608, RRID:AB_10900994

Product name

APC anti-human CD195 (CCR5) Phospho (Ser349)

Antibody type

Monoclonal

Antigen

Phosphopeptide (CEAPERA(pS)(pS)VYTR(pS)TGEQI(pS)VGL) of CCR5

Description

CD195, also known as CCR5, is a 45 kD G protein-coupled seven transmembrane CC-chemokine receptor. It binds to MIP-1α, MIP-1β, and RANTES and is expressed on a subset of T cells and monocytes. CD195 mediates an intracellular signal thought to induce cell differentiation and proliferation. CCR5 has also been shown to act as a co-receptor for R5 HIV-1 cell entry; modification of CCR5 by sulfation contributes to the efficiency of HIV-1 entry.

Reactivity

Human

Host

Mouse

Conjugate

Red dye

Isotype

IgG

Vial size

100 tests

Storage

The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Submitted references

TLR2-dependent pathway of heterologous down-modulation for the CC chemokine receptors 1, 2, and 5 in human blood monocytes.

Fox JM, Letellier E, Oliphant CJ, Signoret N
Blood 2011 Feb 10;117(6):1851-60

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

PMA (100 ng/ml)-stimulated (5 minutes) human peripheral blood lymphocytes surface stained with CD3 FITC and intracellularly stained with CD195 (clone E11/19) APC (top) or mouse IgG1, κ APC isotype control (bottom).

Conjugate

Red dye