66020-1-Ig

antibody from Proteintech Group

Targeting: BCL2L1 Bcl-X, bcl-xL, bcl-xS, BCL2L, BCLX, PPP1R52

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

66020-1-Ig

Provider

Proteintech Group

Proper citation

Proteintech Cat#66020-1-Ig, RRID:AB_11042315

Product name

Bcl-XL antibody

Antibody type

Monoclonal

Description

Bcl-XL antibody (Cat. #66020-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

4C12A6

Vial size

20ul, 150ul

References

Submitted references

Senolytic elimination of therapy-induced senescent cells by ABT-263 improves chemotherapeutic efficacy in esophageal squamous cell carcinoma.

Zhang D, Zhu J, Zou R, Zhang H, Yu BP, Li P, Luo Y, Jiang Z, Hou Y, Zhang JW
Biochemical pharmacology 2026 Mar;245:117659

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Overexpression of Tgm2 in Chinese Hamster Ovary Cells Enhances Recombinant Monoclonal Antibody Expression and Promotes Cell Proliferation through Reduction of Apoptosis.

Zhang HJ, Zhao Q, Zhang M, Yang LL, Abiti J, Han MM, Gao YP, Lu JT, Wang JN, Ji MY, Zhang X, Wang W, Qiu LL, Wang XY, Wang TY, Jia YL
ACS synthetic biology 2025 May 16;14(5):1802-1812

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Dimethyl fumarate abrogates hepatocellular carcinoma growth by inhibiting Nrf2/Bcl-xL axis and enhances sorafenib's efficacy.

Faleti OD, Alsaadawe M, Long J, Luo Q, Hu L, Zhang Y, Deng S, Wu G, Fang W, He M, Lyu X
Scientific reports 2025 May 14;15(1):16724

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Therapy-induced senescence is a transient drug resistance mechanism in breast cancer.

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Molecular cancer 2025 May 1;24(1):128

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Mast cell tryptase-PAR2 axis promotes ovarian fibrosis through RNF152-mediated stabilization of Bcl-xL.

Zhang X, Zhang J, Wang C
Journal of ovarian research 2025 Jun 5;18(1):122

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Discovery of N-Trifluoromethylated Noscapines as Novel and Potent Agents for the Treatment of Glioblastoma.

Cui G, Fan Y, Yang Y, Ma Y, Deng H, Wang P, Zhu Y, Li J, Wei J, Zhang Y
Journal of medicinal chemistry 2025 Jan 9;68(1):247-260

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Chinese medicine 2024 Jan 9;19(1):7

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Zhang X, Tao Y, Xu Z, Jiang B, Yang X, Huang T, Tan W
Biochemical pharmacology 2024 Dec;230(Pt 1):116542

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Interleukin-22 Inhibits Apoptosis of Gingival Epithelial Cells Through TGF-β Signaling Pathway During Periodontitis.

Huang Y, Zhang L, Tan L, Zhang C, Li X, Wang P, Gao L, Zhao C
Inflammation 2023 Oct;46(5):1871-1886

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Anti-apoptotic protein BCL-XL as a therapeutic vulnerability in gastric cancer.

Wei Y, Zhang L, Wang C, Li Z, Luo M, Xie G, Yang X, Li M, Ren S, Zhao D, Gao R, Gong JN
Animal models and experimental medicine 2023 Jun;6(3):245-254

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Targeting proteasomal deubiquitinases USP14 and UCHL5 with b-AP15 reduces 5-fluorouracil resistance in colorectal cancer cells.

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Acta pharmacologica Sinica 2023 Dec;44(12):2537-2548

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Inhibition of proteasomal deubiquitinases USP14 and UCHL5 overcomes tyrosine kinase inhibitor resistance in chronic myeloid leukaemia.

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Mechanism of a new photosensitizer (TBZPy) in the treatment of high-risk human papillomavirus-related diseases.

Li Z, Xiao Z, Feng Y, Wang Q, Teng M
Photodiagnosis and photodynamic therapy 2022 Mar;37:102591

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Ubiquitination of SARS-CoV-2 ORF7a Prevents Cell Death Induced by Recruiting BclXL To Activate ER Stress.

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One-Two Punch Therapy for the Treatment of T-Cell Malignancies Involving p53-Dependent Cellular Senescence.

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Molecular cancer research : MCR 2013 Dec;11(12):1508-20

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig(Bcl-xL antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using BCL2L1 antibody 66020-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue