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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Immunocytochemistry [5]
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- Product number
- MA3-942 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calpain 2 Monoclonal Antibody (107-82)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA3-942 detects the 80 kDa subunit of m-calpain in human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, pig cultured cells, and rabbit samples. This antibody does not cross-react with mu-calpain, n-calpain, calmodulin or calpastatin. MA3-942 has been successfully used in Western blot, IF, immunohistochemistry, and ELISA procedures. By Western blot, this antibody detects an ~80 kDa protein representing m-calpain in bovine platelets. Immunocytochemical staining of m-calpain in LLC-PK1 cells with MA3-942 results in diffuse cytoplasmic staining. This product has not been shown to be effective in immunoprecipitation experiments. The MA3-942 antigen is purified bovine skeletal muscle 80 kDa subunit of m-calpain. This antibody recognizes an epitope between amino acids 502-699 (domain III/IV) of human calpain.
- Reactivity
- Human, Rat, Bovine, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 107-82
- Vial size
- 100 μg
- Concentration
- 1 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references Porcine epidemic diarrhea virus reduces feed efficiency in nursery pigs.
Associations among Protein Biomarkers and pH and Color Traits in Longissimus thoracis and Rectus abdominis Muscles in Protected Designation of Origin Maine-Anjou Cull Cows.
Role of mu-calpain in human decidua for recurrent miscarriage.
Purification and characterization of calpain and calpastatin from rainbow trout, Oncorhynchus mykiss.
Effects of 25-hydroxyvitamin D3 and manipulated dietary cation-anion difference on the tenderness of beef from cull native Korean cows.
Calpain may contribute to hereditary cataract formation in sheep.
Fiber type-specific expression of major proteolytic systems in fast- to slow-transforming rabbit muscle.
Changes in the calpains and calpastatin during postmortem storage of bovine muscle.
Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains.
Curry SM, Burrough ER, Schwartz KJ, Yoon KJ, Lonergan SM, Gabler NK
Journal of animal science 2018 Feb 15;96(1):85-97
Journal of animal science 2018 Feb 15;96(1):85-97
Associations among Protein Biomarkers and pH and Color Traits in Longissimus thoracis and Rectus abdominis Muscles in Protected Designation of Origin Maine-Anjou Cull Cows.
Gagaoua M, Couvreur S, Le Bec G, Aminot G, Picard B
Journal of agricultural and food chemistry 2017 May 3;65(17):3569-3580
Journal of agricultural and food chemistry 2017 May 3;65(17):3569-3580
Role of mu-calpain in human decidua for recurrent miscarriage.
Kumagai K, Ozaki Y, Nakanishi T, Inomata M, Furuno T, Nakanishi M, Ogasawara MS
American journal of reproductive immunology (New York, N.Y. : 1989) 2008 Apr;59(4):339-46
American journal of reproductive immunology (New York, N.Y. : 1989) 2008 Apr;59(4):339-46
Purification and characterization of calpain and calpastatin from rainbow trout, Oncorhynchus mykiss.
Saito M, Li H, Thompson VF, Kunisaki N, Goll DE
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 2007 Apr;146(4):445-55
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 2007 Apr;146(4):445-55
Effects of 25-hydroxyvitamin D3 and manipulated dietary cation-anion difference on the tenderness of beef from cull native Korean cows.
Cho YM, Choi H, Hwang IH, Kim YK, Myung KH
Journal of animal science 2006 Jun;84(6):1481-8
Journal of animal science 2006 Jun;84(6):1481-8
Calpain may contribute to hereditary cataract formation in sheep.
Robertson LJ, Morton JD, Yamaguchi M, Bickerstaffe R, Shearer TR, Azuma M
Investigative ophthalmology & visual science 2005 Dec;46(12):4634-40
Investigative ophthalmology & visual science 2005 Dec;46(12):4634-40
Fiber type-specific expression of major proteolytic systems in fast- to slow-transforming rabbit muscle.
Sultan KR, Dittrich BT, Leisner E, Paul N, Pette D
American journal of physiology. Cell physiology 2001 Feb;280(2):C239-47
American journal of physiology. Cell physiology 2001 Feb;280(2):C239-47
Changes in the calpains and calpastatin during postmortem storage of bovine muscle.
Boehm ML, Kendall TL, Thompson VF, Goll DE
Journal of animal science 1998 Sep;76(9):2415-34
Journal of animal science 1998 Sep;76(9):2415-34
Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains.
Cong J, Thompson VF, Goll DE
The Journal of biological chemistry 1993 Dec 5;268(34):25740-7
The Journal of biological chemistry 1993 Dec 5;268(34):25740-7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of M-Calpain in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody (Product # MA3-942) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). M-Calpain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of M-Calpain in MCF-7 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody (Product # MA3-942) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). M-Calpain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of M-Calpain in U251 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody (Product # MA3-942) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). M-Calpain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of M-Calpain in MCF-7 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody (Product # MA3-942) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). M-Calpain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of M-Calpain in U251 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody (Product # MA3-942) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). M-Calpain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
