Explore
Validate
Learn
Western blot
ELISA
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-14663 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- A2M Monoclonal Antibody (F1-P1C11 #3)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-14663 targets Alpha2-Macroglobulin in ELISA, IP, and RIA applications and shows reactivity with Human samples. The MA5-14663 immunogen is purified human plasma AMG. MA5-14663 detects Alpha2-Macroglobulin which has a predicted molecular weight of approximately 162 kDa. Product MA514663 is a smaller package size of MIA1201 (formerly sold as a Seradyn product).
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- F1-P1C11 #3
- Vial size
- 100 μg
- Concentration
- 1 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Direct ELISA analysis of Alpha2-Macroglobulin was performed by coating wells of a 96-well plate with 100 µL per well of Alpha2-Macroglobulin recombinant protein diluted in carbonate/bicarbonate buffer (Product # 28382), at a concentration of 1 µg/mL overnight at 4C. Wells of the plate were washed, blocked with StartingBlock blocking buffer (Product # 37538), and incubated with 100 µL per well of a mouse Alpha2-Macroglobulin monoclonal antibody (Product # MA5-14663 F1-P1C11 #3) starting at a concentration of 2 µg/mL and serially diluting it to a concentration of 0.03125 µg/mL for 2 hours at room temperature. The plate was washed and incubated with 100 µL per well of an HRP-conjugated goat anti-mouse IgG secondary antibody (Product # 31430) at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate (Product # 34028) for 30 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid and absorbances were read on a spectrophotometer at 450-550 nm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Direct ELISA analysis of Alpha2-Macroglobulin was performed by coating wells of a 96-well plate with 100 µL per well of Alpha2-Macroglobulin recombinant protein diluted in carbonate/bicarbonate buffer (Product # 28382), at a concentration of 1 µg/mL overnight at 4C. Wells of the plate were washed, blocked with StartingBlock blocking buffer (Product # 37538), and incubated with 100 µL per well of a mouse Alpha2-Macroglobulin monoclonal antibody (Product # MA5-14663 F1-P1C11 #3) starting at a concentration of 2 µg/mL and serially diluting it to a concentration of 0.03125 µg/mL for 2 hours at room temperature. The plate was washed and incubated with 100 µL per well of an HRP-conjugated goat anti-mouse IgG secondary antibody (Product # 31430) at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate (Product # 34028) for 30 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid and absorbances were read on a spectrophotometer at 450-550 nm.
