MA5-15073

antibody from Invitrogen Antibodies

Targeting: MAP3K7 MEKK7, TAK1

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15073

Provider

Invitrogen Antibodies

Product name

Phospho-TAK1 (Thr184, Thr187) Monoclonal Antibody (K.846.3)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

K.846.3

Vial size

100 μL

Concentration

24 μg/mL

Storage

-20°C

References

Submitted references

Albumin inhibits the nuclear translocation of Smad3 via interleukin-1beta signaling in hepatic stellate cells.

Park JH, Kim J, Choi SY, Lee B, Lee JE, Park H, Moon JW, Park SH, Lee JM, Lee HS, Oh J
Scientific reports 2021 Feb 4;11(1):3196

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TAK1 mediates neuronal pyroptosis in early brain injury after subarachnoid hemorrhage.

Xu P, Tao C, Zhu Y, Wang G, Kong L, Li W, Li R, Li J, Zhang C, Wang L, Liu X, Sun W, Hu W
Journal of neuroinflammation 2021 Aug 30;18(1):188

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Toll-Like Receptor 9-Dependent AMPKα Activation Occurs via TAK1 and Contributes to RhoA/ROCK Signaling and Actin Polymerization in Vascular Smooth Muscle Cells.

McCarthy CG, Wenceslau CF, Ogbi S, Szasz T, Webb RC
The Journal of pharmacology and experimental therapeutics 2018 Apr;365(1):60-71

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TAK1 modulates satellite stem cell homeostasis and skeletal muscle repair.

Ogura Y, Hindi SM, Sato S, Xiong G, Akira S, Kumar A
Nature communications 2015 Dec 9;6:10123

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 R-III treatment induces the phosphorylation of the Smad3 linker region IL-1beta-dependently. ( A ) Hepatic stellate cells after passage 1 (HSCs-P1) were treated with R-III (0.25 muM) for 20 h, and the cell lysates were then analyzed by western blotting. The quantitative densitometric data are presented as mean fold change +- SD of three independent experiments. P -value, paired t -test (compared with untreated cells). * P < 0.05, ** P < 0.01 ( B ) HSCs-P1 were treated with R-III in the presence or absence of interleukin (IL)-1RA (1 mug/mL), and the cell lysates were then analyzed by western blotting. ( C ) HSCs-P1 were treated with R-III in the presence of an inhibitor for TAK1 ((5Z)-7-oxozeaenol, 0.25 muM), p38 (SB203580, 5 muM), or JNK (SP600125, 10 muM), and the cell lysates were then analyzed by western blotting. alpha-tubulin was used as a loading control. Full-length blots are presented in Supplementary Fig. S4.