PA5-17725

antibody from Invitrogen Antibodies

Targeting: ACACA ACAC, ACC, ACC1

Western blot

Immunoprecipitation

Antibody data

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Validation data

ACACA protein structure - PA5-17725 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Phospho-Acetyl-CoA Carboxylase (Ser79) was performed using 70% confluent log phase HeLa cells treated with Heat Shock (44 degrees for 3 hr). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Acetyl-CoA Carboxylase (Ser79) Polyclonal Antibody (Product # PA5-17725) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing increased expression in cytoplasm. Panel e represents the control cells showing weak cytoplasmic staining. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Phospho-Acetyl-CoA Carboxylase (Ser79) was performed using 70% confluent log phase HeLa cells treated with Heat Shock (44 degrees for 3 hr). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Acetyl-CoA Carboxylase (Ser79) Polyclonal Antibody (Product # PA5-17725) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing increased expression in cytoplasm. Panel e represents the control cells showing weak cytoplasmic staining. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.