PA5-21386

antibody from Invitrogen Antibodies

Targeting: DDT D-DT, DDCT, MIF-2, MIF2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21386

Provider

Invitrogen Antibodies

Product name

DDT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: 293T, HeLa, Raji, mouse liver, rat liver. Predicted reactivity: Pig (80%), Rhesus Monkey (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat, Canine

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using DDT Polyclonal Antibody (Product # PA5-21386). Sample (30 µg of whole cell lysate). Lane A: Raji . 15% SDS PAGE. DDT Polyclonal Antibody (Product # PA5-21386) diluted at 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DDT was performed by separating 50 µg of mouse tissue extract by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a DDT Polyclonal Antibody (Product # PA5-21386) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DDT was performed by separating 50 µg of rat tissue extract by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a DDT Polyclonal Antibody (Product # PA5-21386) at a dilution of 1:500.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on canine epididymis tissue sections. Endogenous peroxidases were blocked by incubating the tissue in 3% hydrogen peroxide for 15 minutes at room temperature. Tissues were blocked, and probed with a DDT polyclonal antibody (Product # PA5-21386) at a dilution of 1:200 overnight at 4C (right panel). Detection was performed using a biotinylated goat anti-rabbit IgG secondary antibody at a dilution of 1:2000 followed by Streptavidin-HRP and DAB substrate. Tissues were visualized by light microscopy. As a negative control, tissues were stained with detection reagents alone (left panel). Data courtesy of the Innovators Program.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using DDT (Product # PA5-21386) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using DDT (Product # PA5-21386) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.