PA5-22064

antibody from Invitrogen Antibodies

Targeting: FH

Western blot

Antibody data

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Validation data

Product number: PA5-22064 - Provider product page
Provider: Invitrogen Antibodies
Product name: Fumarase Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Recommended positive controls: 293T, A431, HeLa, HepG2, mouse liver, rat liver. Predicted reactivity: Rat (98%), Zebrafish (90%), Pig (98%), Chicken (91%), Sheep (99%), Chimpanzee (99%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 μL
Concentration: 1.49 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

FH protein structure - PA5-22064 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of fumarate hydratase showing staining in the mitochondria of HeLa cells. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min and stained using a fumarate hydratase polyclonal antibody (Product # PA5-22064) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of Fumarase was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: Fumarase Polyclonal Antibody (Product # PA5-22064) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fumarase Polyclonal Antibody detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Fumarate hydratase stained by Fumarase Polyclonal Antibody (Product # PA5-22064) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fumarase Polyclonal Antibody detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Fumarate hydratase stained by Fumarase Polyclonal Antibody (Product # PA5-22064) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of Fumarase was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: Fumarase Polyclonal Antibody (Product # PA5-22064) diluted at 1:500. Blue: Hoechst 33342 staining.