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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- NBP1-60140 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal F4/80 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.1 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The Angiotensin Receptor Blocker Losartan Suppresses Growth of Pulmonary Metastases via AT1R-Independent Inhibition of CCR2 Signaling and Monocyte Recruitment.
Advax4 delta inulin combination adjuvant together with ECMX, a fusion construct of four protective mTB antigens, induces a potent Th1 immune response and protects mice against Mycobacterium tuberculosis infection.
A Broad Blockade of Signaling from the IL-20 Family of Cytokines Potently Attenuates Collagen-Induced Arthritis.
Dual 12/15- and 5-lipoxygenase deficiency in macrophages alters arachidonic acid metabolism and attenuates peritonitis and atherosclerosis in ApoE knock-out mice.
Gr-1(+)CD11b(+) cells as an accelerator of sepsis stemming from Pseudomonas aeruginosa wound infection in thermally injured mice.
Inactivation of the F4/80 glycoprotein in the mouse germ line.
The inflammatory infiltrate in the acute stage of the dextran sulphate sodium induced colitis: B cell response differs depending on the percentage of DSS used to induce it.
The inflammatory infiltrate in the acute stage of the dextran sulphate sodium induced colitis: B cell response differs depending on the percentage of DSS used to induce it.
Macrophage trafficking in the uterus and cervix precedes parturition in the mouse.
Markers of mouse macrophage development detected by monoclonal antibodies.
A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes.
Regan DP, Coy JW, Chahal KK, Chow L, Kurihara JN, Guth AM, Kufareva I, Dow SW
Journal of immunology (Baltimore, Md. : 1950) 2019 May 15;202(10):3087-3102
Journal of immunology (Baltimore, Md. : 1950) 2019 May 15;202(10):3087-3102
Advax4 delta inulin combination adjuvant together with ECMX, a fusion construct of four protective mTB antigens, induces a potent Th1 immune response and protects mice against Mycobacterium tuberculosis infection.
de Paula Oliveira Santos B, Trentini MM, Machado RB, Rúbia Nunes Celes M, Kipnis A, Petrovsky N, Junqueira-Kipnis AP
Human vaccines & immunotherapeutics 2017 Dec 2;13(12):2967-2976
Human vaccines & immunotherapeutics 2017 Dec 2;13(12):2967-2976
A Broad Blockade of Signaling from the IL-20 Family of Cytokines Potently Attenuates Collagen-Induced Arthritis.
Liu X, Zhou H, Huang X, Cui J, Long T, Xu Y, Liu H, Yu R, Zhao R, Luo G, Huang A, Liang JG, Liang P
Journal of immunology (Baltimore, Md. : 1950) 2016 Oct 15;197(8):3029-3037
Journal of immunology (Baltimore, Md. : 1950) 2016 Oct 15;197(8):3029-3037
Dual 12/15- and 5-lipoxygenase deficiency in macrophages alters arachidonic acid metabolism and attenuates peritonitis and atherosclerosis in ApoE knock-out mice.
Poeckel D, Zemski Berry KA, Murphy RC, Funk CD
The Journal of biological chemistry 2009 Jul 31;284(31):21077-89
The Journal of biological chemistry 2009 Jul 31;284(31):21077-89
Gr-1(+)CD11b(+) cells as an accelerator of sepsis stemming from Pseudomonas aeruginosa wound infection in thermally injured mice.
Kobayashi M, Yoshida T, Takeuchi D, Jones VC, Shigematsu K, Herndon DN, Suzuki F
Journal of leukocyte biology 2008 Jun;83(6):1354-62
Journal of leukocyte biology 2008 Jun;83(6):1354-62
Inactivation of the F4/80 glycoprotein in the mouse germ line.
Schaller E, Macfarlane AJ, Rupec RA, Gordon S, McKnight AJ, Pfeffer K
Molecular and cellular biology 2002 Nov;22(22):8035-43
Molecular and cellular biology 2002 Nov;22(22):8035-43
The inflammatory infiltrate in the acute stage of the dextran sulphate sodium induced colitis: B cell response differs depending on the percentage of DSS used to induce it.
Stevceva L, Pavli P, Husband AJ, Doe WF
BMC clinical pathology 2001;1(1):3
BMC clinical pathology 2001;1(1):3
The inflammatory infiltrate in the acute stage of the dextran sulphate sodium induced colitis: B cell response differs depending on the percentage of DSS used to induce it.
Stevceva L, Pavli P, Husband AJ, Doe WF
BMC clinical pathology 2001;1(1):3
BMC clinical pathology 2001;1(1):3
Macrophage trafficking in the uterus and cervix precedes parturition in the mouse.
Mackler AM, Iezza G, Akin MR, McMillan P, Yellon SM
Biology of reproduction 1999 Oct;61(4):879-83
Biology of reproduction 1999 Oct;61(4):879-83
Markers of mouse macrophage development detected by monoclonal antibodies.
Leenen PJ, de Bruijn MF, Voerman JS, Campbell PA, van Ewijk W
Journal of immunological methods 1994 Sep 14;174(1-2):5-19
Journal of immunological methods 1994 Sep 14;174(1-2):5-19
A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes.
Malorny U, Michels E, Sorg C
Cell and tissue research 1986;243(2):421-8
Cell and tissue research 1986;243(2):421-8
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: F4/80 Antibody (BM8) [NBP1-60140] - Expression on macrophages in mouse liver. Staining of frozen tissue section with antibody BM8.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: F4/80 Antibody (BM8) [NBP1-60140] - Red: F4/80, Green: CD31/PECAM-1, Blue: DAPI+ nuclei. Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with PE-conjugated F4/80 and Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) for 2 hours at the room temperature. This image was submitted via a customer review. Image from the PE version of this antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Analysis using the FITC conjugate of NBP1-60140. Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 FITC.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Splenocytes isolated from a IL1R-/- mouse (IL1b receptor KO) were stained with F4/80 Antibody (BM8) and CD45-e450 (1:100 in FACS buffer) for 30 minutes. Cells were then washed and analyzed by flow cytometry. Left panel shows the isotype control. This image was submitted via a customer review. Image from the PE version of this antibody.
