Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-41186 - Provider product page

- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-41186, RRID:AB_10005036
- Product name
- Mouse Monoclonal BRCA1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references A synthetic lethal siRNA screen identifying genes mediating sensitivity to a PARP inhibitor.
Epigenetic inactivation of the chromosomal stability control genes BRCA1, BRCA2, and XRCC5 in non-small cell lung cancer.
BRCA1 gene expression in breast cancer: a correlative study between real-time RT-PCR and immunohistochemistry.
Differential expressions of BRCA1 and BRCA2 in infantile gynecomastia.
BRCA1 shifts p53-mediated cellular outcomes towards irreversible growth arrest.
Cell cycle differences in DNA damage-induced BRCA1 phosphorylation affect its subcellular localization.
The prognostic impact of hormone receptors and c-erbB-2 in pregnancy-associated breast cancer and their correlation with BRCA1 and cell cycle modulators.
BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite.
BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite.
Turner NC, Lord CJ, Iorns E, Brough R, Swift S, Elliott R, Rayter S, Tutt AN, Ashworth A
The EMBO journal 2008 May 7;27(9):1368-77
The EMBO journal 2008 May 7;27(9):1368-77
Epigenetic inactivation of the chromosomal stability control genes BRCA1, BRCA2, and XRCC5 in non-small cell lung cancer.
Lee MN, Tseng RC, Hsu HS, Chen JY, Tzao C, Ho WL, Wang YC
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Feb 1;13(3):832-8
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Feb 1;13(3):832-8
BRCA1 gene expression in breast cancer: a correlative study between real-time RT-PCR and immunohistochemistry.
Al-Mulla F, Abdulrahman M, Varadharaj G, Akhter N, Anim JT
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2005 May;53(5):621-9
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2005 May;53(5):621-9
Differential expressions of BRCA1 and BRCA2 in infantile gynecomastia.
Bernard-Gallon DJ, Dechelotte PJ, Le Corre L, Chalabi N, Vissac-Sabatier C, Bignon YJ
Anticancer research 2004 Jan-Feb;24(1):321-4
Anticancer research 2004 Jan-Feb;24(1):321-4
BRCA1 shifts p53-mediated cellular outcomes towards irreversible growth arrest.
Ongusaha PP, Ouchi T, Kim KT, Nytko E, Kwak JC, Duda RB, Deng CX, Lee SW
Oncogene 2003 Jun 12;22(24):3749-58
Oncogene 2003 Jun 12;22(24):3749-58
Cell cycle differences in DNA damage-induced BRCA1 phosphorylation affect its subcellular localization.
Okada S, Ouchi T
The Journal of biological chemistry 2003 Jan 17;278(3):2015-20
The Journal of biological chemistry 2003 Jan 17;278(3):2015-20
The prognostic impact of hormone receptors and c-erbB-2 in pregnancy-associated breast cancer and their correlation with BRCA1 and cell cycle modulators.
Reed W, Sandstad B, Holm R, Nesland JM
International journal of surgical pathology 2003 Apr;11(2):65-74
International journal of surgical pathology 2003 Apr;11(2):65-74
BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite.
Bernard-Gallon DJ, Déchelotte P, Vissac C, Aunoble B, Cravello L, Malpuech G, Bignon YJ
Breast cancer research : BCR 2001;3(1):61-5
Breast cancer research : BCR 2001;3(1):61-5
BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite.
Bernard-Gallon DJ, Déchelotte P, Vissac C, Aunoble B, Cravello L, Malpuech G, Bignon YJ
Breast cancer research : BCR 2001;3(1):61-5
Breast cancer research : BCR 2001;3(1):61-5
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image

- Experimental details
- Western Blot: BRCA1 Antibody (8F7) [NBP1-41186] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image

- Experimental details
- Immunohistochemistry: BRCA1 Antibody (8F7) [NBP1-41186] - Paraffin-embedded Breast ca, using BRCA1 antibody at 1:200 dilution.