NBP1-86168

antibody from Novus Biologicals

Targeting: CD14

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-86168

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-86168, RRID:AB_11040267

Product name

Rabbit Polyclonal CD14 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human CD14 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Increased CTLA-4(+) T cells and an increased ratio of monocytes with loss of class II (CD14(+) HLA-DR(lo/neg)) found in aggressive pediatric sarcoma patients.

Hingorani P, Maas ML, Gustafson MP, Dickman P, Adams RH, Watanabe M, Eshun F, Williams J, Seidel MJ, Dietz AB
Journal for immunotherapy of cancer 2015;3:35

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LPS and IL-1 differentially activate mouse and human astrocytes: role of CD14.

Tarassishin L, Suh HS, Lee SC
Glia 2014 Jun;62(6):999-1013

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High-density lipoprotein endocytosis in endothelial cells.

Fruhwürth S, Pavelka M, Bittman R, Kovacs WJ, Walter KM, Röhrl C, Stangl H
World journal of biological chemistry 2013 Nov 26;4(4):131-40

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Functional contribution of elevated circulating and hepatic non-classical CD14CD16 monocytes to inflammation and human liver fibrosis.

Zimmermann HW, Seidler S, Nattermann J, Gassler N, Hellerbrand C, Zernecke A, Tischendorf JJ, Luedde T, Weiskirchen R, Trautwein C, Tacke F
PloS one 2010 Jun 10;5(6):e11049

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CD14 Antibody [NBP1-86168] - Analysis in control (vector only transfected HEK293T lysate) and CD14 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CD14 Antibody [NBP1-86168] - Analysis using Anti-CD14 antibody NBP1-86168 (A) shows similar pattern to independent antibody NBP1-86166 (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human liver, lymph node, pancreas and rectum using Anti-CD14 antibody NBP1-86168 (A) shows similar protein distribution across tissues to independent antibody NBP1-86166 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Analysis in human lymph node and pancreas tissues. Corresponding CD14 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human rectum shows moderate to strong cytoplasmic positivity in lymphoid cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human lymph node shows moderate to strong membranous positivity in lymphoid cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human liver shows moderate to strong membranous positivity in hepatic sinusoid cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86168] - Staining of human tonsil shows moderate membranous positivity in germinal center cells.