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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [7]
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- Product number
- NBP1-90246 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-90246, RRID:AB_11007792
- Product name
- Rabbit Polyclonal APP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human APP antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Candidate serological biomarkers for cancer identified from the secretomes of 23 cancer cell lines and the human protein atlas.
Wu CC, Hsu CW, Chen CD, Yu CJ, Chang KP, Tai DI, Liu HP, Su WH, Chang YS, Yu JS
Molecular & cellular proteomics : MCP 2010 Jun;9(6):1100-17
Molecular & cellular proteomics : MCP 2010 Jun;9(6):1100-17
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: APP Antibody [NBP1-90246] - Analysis in control (vector only transfected HEK293T lysate) and APP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: APP Antibody [NBP1-90246] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: APP Antibody [NBP1-90246] - Staining of human tonsil shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: APP Antibody [NBP1-90246] - Staining of human cerebellum shows moderate granular positivity in cytoplasm in Purkinje cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: APP Antibody [NBP1-90246] - Staining of human cerebral cortex shows moderate granular positivity in cytoplasm in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: APP Antibody [NBP1-90246] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: APP Antibody [NBP1-90246] - Staining of human hippocampus shows moderate granular positivity in cytoplasm in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: APP Antibody [NBP1-90246] - Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding APP RNA-seq data are presented for the same tissues.
