PA5-21419

antibody from Invitrogen Antibodies

Targeting: CD44 CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1

Western blot

Immunoprecipitation

Antibody data

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Validation data

Product number: PA5-21419 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD44 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Recommended positive controls: A549, H1299, HeLa, A431, MDA-MB-231, MDA-MB-231, MDA-MB-231 (24 µg/mL Tunicamycin treatment for 16 hr), HeLa (12 µg/mL Tunicamycin treatment for 48 hr), HeLa (12 µg/mL Tunicamycin treatment for 48 hr and shCD44-transfected 293T). Predicted reactivity: Dog (82%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Rat, Rabbit
Host: Rabbit
Isotype: IgG
Vial size: 100 μL
Concentration: 0.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CD44 protein structure - PA5-21419 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CD44/H-CAM in methanol-fixed HeLa cells using a CD44/H-CAM polyclonal antibody (Product # PA5-21419) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using CD44 antibody (Product # PA5-21419) (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: CD44 Polyclonal Antibody detects CD44 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: CD44 stained by CD44 Polyclonal Antibody (Product # PA5-21419) diluted at 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using CD44 antibody (Product # PA5-21419) (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: CD44 Polyclonal Antibody detects CD44 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: CD44 stained by CD44 Polyclonal Antibody (Product # PA5-21419) diluted at 1:500.