MA5-15651

antibody from Invitrogen Antibodies

Targeting: FLT4 PCL, VEGFR-3, VEGFR3

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15651

Provider

Invitrogen Antibodies

Product name

VEGF Receptor 3 Monoclonal Antibody (4H4)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15651 targets FLT4 in indirect ELISA, WB applications and shows reactivity with Human samples. The MA5-15651 immunogen is purified recombinant fragment of human FLT4 expressed in E. Coli.. MA5-15651 detects FLT4 which has a predicted molecular weight of approximately 145kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4H4

Vial size

100 μL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Vascular endothelial growth factor receptor 3 was performed using 70% confluent log phase HMEC-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with VEGF Receptor 3 Monoclonal Antibody (4H4) (Product # MA5-15651) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained withRhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing cytoplasmic localization. Panel e represents Low expression in HUVEC. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification in EVOS™ M7000 Imaging System (Product # AMF7000) and externally deconvoluted (D.Sage et al. / Methods 115 (2017) 28-41).