PA5-29813

antibody from Invitrogen Antibodies

Targeting: HADHA GBP, LCEH, LCHAD, MTPA

Western blot

Antibody data

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Validation data

Product number: PA5-29813 - Provider product page
Provider: Invitrogen Antibodies
Product name: HADHA Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Recommended positive controls: 293T, A431, mouse liver, PC-12. Predicted reactivity: Mouse (86%), Rat (86%), Pig (92%), Sheep (89%), Rhesus Monkey (97%), Bovine (89%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 μL
Concentration: 0.37 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

HADHA protein structure - PA5-29813 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of HADHA was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HADHA Polyclonal Antibody (Product # PA5-29813) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Mitochondria (Panel c: red) was stained with Mitotracker Red CMXRos (Product # M7512). Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of HADHA was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HADHA Polyclonal Antibody (Product # PA5-29813) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HADHA Polyclonal Antibody detects HADHA protein at Mitochondia by immunofluorescent analysis. Sample: HepG2 cells were fixed in -20ºC 100% MeOH for 5 min. Green: HADHA protein stained by HADHA Polyclonal Antibody (Product # PA5-29813) diluted at 1:500. Blue: Hoechst 33343 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of HADHA was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HADHA Polyclonal Antibody (Product # PA5-29813) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HADHA Polyclonal Antibody detects HADHA protein at Mitochondia by immunofluorescent analysis. Sample: HepG2 cells were fixed in -20ºC 100% MeOH for 5 min. Green: HADHA protein stained by HADHA Polyclonal Antibody (Product # PA5-29813) diluted at 1:500. Blue: Hoechst 33343 staining.