PA5-16895

antibody from Invitrogen Antibodies

Targeting: ENG CD105, END, HHT1, ORW, ORW1

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-16895

Provider

Invitrogen Antibodies

Product name

Anti-CD105 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

PA5-16899 targets Fli-1 in WB applications and shows reactivity with Human, mouse, and Rat samples. The PA5-16899 immunogen is synthetic peptide derived from C-terminus of human Fli-1.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

500 µL

Concentration

0.2 mg/ml

Storage

4° C

References

Submitted references

Inhibition of hypoxia inducible factor 1 and topoisomerase with acriflavine sensitizes perihilar cholangiocarcinomas to photodynamic therapy.

Weijer R, Broekgaarden M, Krekorian M, Alles LK, van Wijk AC, Mackaaij C, Verheij J, van der Wal AC, van Gulik TM, Storm G, Heger M
Oncotarget 2016 Jan 19;7(3):3341-56

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Deletion of IL-33R attenuates VEGF expression and enhances necrosis in mammary carcinoma.

Milosavljevic MZ, Jovanovic IP, Pejnovic NN, Mitrovic SL, Arsenijevic NN, Simovic Markovic BJ, Lukic ML
Oncotarget 2016 Apr 5;7(14):18106-15

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Early onset of endothelial cell proliferation in coronary thrombi of patients with an acute myocardial infarction: implications for plaque healing.

Li X, Kramer MC, VAN DER Loos CM, Ploegmakers HJ, DE Boer OJ, Koch KT, Tijssen JG, DE Winter RJ, VAN DER Wal AC
Journal of thrombosis and haemostasis : JTH 2012 Mar;10(3):466-73

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CD106 (green) showing staining in the cytoplasm and membrane of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD106 (Product # PA5-16895) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human tonsil stained with anti-CD105 using peroxidase conjugate and AEC chromogen. Note cytoplasmic staining of endothelial cells.