PA5-13658

antibody from Invitrogen Antibodies

Targeting: CCL2 GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-13658

Provider

Invitrogen Antibodies

Product name

MCP-1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with non-human primate based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 μL

Concentration

2.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis using a CCL2 polyclonal antibody (Product # PA5-13658) in HeLa cells. 0.025 mg/mL primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. Red counterstaining is PI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using a CCL2 polyclonal antibody (Product # PA5-13658) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of MCP-1 in HeLa cells. Samples were incubated with MCP-1 polyclonal antibody (Product # PA5-13658) using a dilution of 0.025 mg/mL followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. Red counterstaining is PI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of MCP-1 in HeLa cells. Samples were incubated in MCP-1 polyclonal antibody (Product # PA5-13658) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MCP-1 in formalin-fixed and paraffin-embedded human brain tissue. Samples were incubated with MCP-1 polyclonal antibody (Product # PA5-13658) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of HeLa cells using a CCL2 polyclonal antibody (Product # PA5-13658) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of MCP-1 in Hela cells (bottom histogram). Samples were incubated with MCP-1 polyclonal antibody (Product # PA5-13658) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (top histogram).