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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- NB500-161 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-161, RRID:AB_10000523
- Product name
- Rabbit Polyclonal KAT3B/p300 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Dynamic Acetylation of Phosphoenolpyruvate Carboxykinase Toggles Enzyme Activity between Gluconeogenic and Anaplerotic Reactions.
Genome-wide analysis of HIF-2α chromatin binding sites under normoxia in human bronchial epithelial cells (BEAS-2B) suggests its diverse functions.
PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia.
Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1.
mTORC1-activated S6K1 phosphorylates Rictor on threonine 1135 and regulates mTORC2 signaling.
Latorre-Muro P, Baeza J, Armstrong EA, Hurtado-Guerrero R, Corzana F, Wu LE, Sinclair DA, López-Buesa P, Carrodeguas JA, Denu JM
Molecular cell 2018 Sep 6;71(5):718-732.e9
Molecular cell 2018 Sep 6;71(5):718-732.e9
Genome-wide analysis of HIF-2α chromatin binding sites under normoxia in human bronchial epithelial cells (BEAS-2B) suggests its diverse functions.
Lee MC, Huang HJ, Chang TH, Huang HC, Hsieh SY, Chen YS, Chou WY, Chiang CH, Lai CH, Shiau CY
Scientific reports 2016 Jul 4;6:29311
Scientific reports 2016 Jul 4;6:29311
PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia.
Luo W, Chen I, Chen Y, Alkam D, Wang Y, Semenza GL
Oncotarget 2016 Feb 9;7(6):6379-97
Oncotarget 2016 Feb 9;7(6):6379-97
Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1.
Luo W, Hu H, Chang R, Zhong J, Knabel M, O'Meally R, Cole RN, Pandey A, Semenza GL
Cell 2011 May 27;145(5):732-44
Cell 2011 May 27;145(5):732-44
mTORC1-activated S6K1 phosphorylates Rictor on threonine 1135 and regulates mTORC2 signaling.
Julien LA, Carriere A, Moreau J, Roux PP
Molecular and cellular biology 2010 Feb;30(4):908-21
Molecular and cellular biology 2010 Feb;30(4):908-21
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KAT3B/p300 Antibody [NB500-161] - Analysis of HeLa lysates using NB500-161. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KAT3B/p300 Antibody [NB500-161] - Nuclear (NE) or cytosolic (S100) extract (10 mg) from HeLa cells. Antibody used at 20 ug/10 mg lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KAT3B/p300 Antibody [NB500-161] - Detection of Human p300 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-p300 antibody NB500-161 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NB500-161] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- p300 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
