PA5-12093

antibody from Invitrogen Antibodies

Targeting: SYVN1 DER3, HRD1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-12093

Provider

Invitrogen Antibodies

Product name

HRD1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 μL

Concentration

1.8 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using a SYVN1 polyclonal antibody (Product # PA5-12093). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with a SYVN1 polyclonal antibody (Product # PA5-12093) (1:200, 2 hr at room temperature). Primary antibody detected by fluor-conjugated donkey anti-rabbit secondary antibody (green) was used (1:1000, 1hr). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/mL, 5 min).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of HRD1 in HeLa cells. Samples were incubated with HRD1 polyclonal antibody (Product # PA5-12093) using a dilution of 1:200 for 2 h at room temperature followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:1,000 for 1hr. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.2%, 30 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/mL, 5 min).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HRD1 in formalin-fixed and paraffin-embedded human Liver tissue. Samples were incubated with HRD1 polyclonal antibody (Product # PA5-12093) which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HRD1 in formalin-fixed and paraffin-embedded human cancer tissue. Samples were incubated with HRD1 polyclonal antibody (Product # PA5-12093) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.