PA5-14215

antibody from Invitrogen Antibodies

Targeting: CD163 M130, MM130, SCARI1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-14215

Provider

Invitrogen Antibodies

Product name

CD163 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 μL

Concentration

2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Iron-Overload triggers ADAM-17 mediated inflammation in Severe Alcoholic Hepatitis.

Maras JS, Das S, Sharma S, Sukriti S, Kumar J, Vyas AK, Kumar D, Bhat A, Yadav G, Choudhary MC, Sharma S, Kumar G, Bihari C, Trehanpati N, Maiwall R, Sarin SK
Scientific reports 2018 Jul 6;8(1):10264

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CD163 in formalin-fixed and paraffin-embedded human hepatocarcinoma. Samples were incubated with CD163 polyclonal antibody (Product # PA5-14215) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Increased CD163 and ADAM17 expression is associated to iron-overload in SAH. ( A ) Workflow and summary of the experiments performed and number of samples used in target determination, and validation experiments including the In-vivo and In-vitro analysis. ( B ) Venn-diagram for unique and shared genes (p < 0.05, fold change >1.5) in the liver and PBMC transcriptome of Group A: SAH-IO.16 shared genes in the liver and PBMC were associated to cellular iron ion homeostasis, cytokine receptor binding and notch signaling. ( C ) Venn-diagram for unique and shared genes identified in the univariate and multivariate projection analysis for the liver and PBMC transcriptome of Group A: SAH-IO documenting CD163, ADAM17 and TMED7 significantly associated to systemic iron overload. ( D ) Immuno-histochemistry (IHC) for iron deposition (Perl's-staining) and expression of ADAM17, CD163, ADAM10 in Group A: SAH-IO (n = 5) vs. Group B: SAH-NIO (n = 5). [For all IHC analysis relative quantization of positively stained cells are expressed as mean number of positive cells/10 high power field (40x)]. ( E ) Perl's staining and expression of CD68, ADAM17, CD163 and ADAM10 in Group A: SAH-IO (n = 5) vs. AC (n = 5). ( F ) Perl's staining, and expression of ADAM17, CD163 and ADAM10 in Thalassemia (n = 3) patients compared to Group A and B.