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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- PA5-18145 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CARD11 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine and mouse based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 μg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CARD11 using CARD11 Polyclonal Antibody (Product # PA5-18145) (1 µg/mL) in staining of Human Jurkat cells (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CARD11 in human spleen. Samples were incubated with CARD11 polyclonal antibody (Product # PA5-18145) using a dilution of 2 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (PFA fixed) analysis of CARD11 using CARD11 Polyclonal Antibody (Product # PA5-18145) (2 µg/mL) in staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CARD11 in human spleen. Samples were incubated with CARD11 polyclonal antibody (Product # PA5-18145) using a dilution of 2 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
